Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	A family of archaeal hibernation factors that bind in tandem and protect ribosomes in dormant cells.
Journal, issue, pages	bioRxiv, Year 2026
Publish date	Jan 19, 2026
Authors	Amos J Nissley / Madison C Williams / Yekaterina Shulgina / Roan W Kivimae / Dipti D Nayak / Jamie H D Cate /
PubMed Abstract	Under nutrient limitation or stress, ribosome hibernation factors inactivate and protect ribosomes. Although ribosome hibernation plays an important role in microbes, we lack a complete understanding ...Under nutrient limitation or stress, ribosome hibernation factors inactivate and protect ribosomes. Although ribosome hibernation plays an important role in microbes, we lack a complete understanding of this process in archaea. Here, we identify a family of hibernation factors, which we designate as single ribosomal subunit inhibitors (SriA-SriD), from the methanogenic archaeon . All four genes are encoded in an operon and each Sri protein inhibits protein synthesis . Deletion of genes in impaired growth recovery after prolonged stationary phase and also led to depletion of the small ribosomal subunit. Cryo-EM structures show that Sri proteins bind to the ribosome in tandem and form conserved protein-protein interfaces. Sri is broadly distributed across archaeal phyla and genes frequently co-occur. Together, these findings establish Sri proteins as a distinct group of hibernation factors that protect ribosomes during dormancy and expand our understanding of ribosome hibernation in archaea.
External links	bioRxiv / PubMed:41867869 / PubMed Central
Methods	EM (single particle)
Resolution	1.94 - 2.96 Å
Structure data	74446 9znf EMDB-74446: Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB composite map PDB-9znf: Cryo-EM structure of the Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB Method: EM (single particle) / Resolution: 2.03 Å 74447 9zng EMDB-74447: Methanosarcina acetivorans 70S ribosome in complex with SriD composite map PDB-9zng: Cryo-EM structure of the Methanosarcina acetivorans 70S ribosome in complex with SriD Method: EM (single particle) / Resolution: 2.06 Å 74448 9zni EMDB-74448: Methanosarcina acetivorans 70S ribosome in complex with SriC and SriD composite map PDB-9zni: Cryo-EM structure of the Methanosarcina acetivorans 70S ribosome in complex with SriC and SriD Method: EM (single particle) / Resolution: 2.89 Å EMDB-74841: Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB consensus map Method: EM (single particle) / Resolution: 2.03 Å EMDB-74851: Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB 50S focused refinement Method: EM (single particle) / Resolution: 1.97 Å EMDB-74853: Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB 30S focused refinement Method: EM (single particle) / Resolution: 2.32 Å EMDB-74854: Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB 30S head focused refinement Method: EM (single particle) / Resolution: 2.48 Å EMDB-74982: Methanosarcina acetivorans 70S ribosome in complex with SriD consensus map Method: EM (single particle) / Resolution: 2.06 Å EMDB-74984: Methanosarcina acetivorans 70S ribosome in complex with SriD 50S focused refinement Method: EM (single particle) / Resolution: 1.94 Å EMDB-74985: Methanosarcina acetivorans 70S ribosome in complex with SriD 30S focused refinement Method: EM (single particle) / Resolution: 2.27 Å EMDB-74988: Methanosarcina acetivorans 70S ribosome in complex with SriC and SriD consensus map Method: EM (single particle) / Resolution: 2.89 Å EMDB-74989: Methanosarcina acetivorans 70S ribosome in complex with SriC and SriD 50S focused refinement Method: EM (single particle) / Resolution: 2.69 Å EMDB-74990: Methanosarcina acetivorans 70S ribosome in complex with SriC and SriD 30S focused refinement Method: EM (single particle) / Resolution: 2.92 Å EMDB-74992: Methanosarcina acetivorans 70S ribosome in complex with SriC and SriD 30S head focused refinement Method: EM (single particle) / Resolution: 2.96 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-HOH: WATER
Source	methanosarcina acetivorans (archaea)
Keywords	RIBOSOME / Hibernation / Archaea