EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A family of archaeal hibernation factors that bind in tandem and protect ribosomes in dormant cells.
ジャーナル・号・ページ	bioRxiv, Year 2026
掲載日	2026年1月19日
著者	Amos J Nissley / Madison C Williams / Yekaterina Shulgina / Roan W Kivimae / Dipti D Nayak / Jamie H D Cate /
PubMed 要旨	Under nutrient limitation or stress, ribosome hibernation factors inactivate and protect ribosomes. Although ribosome hibernation plays an important role in microbes, we lack a complete understanding ...Under nutrient limitation or stress, ribosome hibernation factors inactivate and protect ribosomes. Although ribosome hibernation plays an important role in microbes, we lack a complete understanding of this process in archaea. Here, we identify a family of hibernation factors, which we designate as single ribosomal subunit inhibitors (SriA-SriD), from the methanogenic archaeon . All four genes are encoded in an operon and each Sri protein inhibits protein synthesis . Deletion of genes in impaired growth recovery after prolonged stationary phase and also led to depletion of the small ribosomal subunit. Cryo-EM structures show that Sri proteins bind to the ribosome in tandem and form conserved protein-protein interfaces. Sri is broadly distributed across archaeal phyla and genes frequently co-occur. Together, these findings establish Sri proteins as a distinct group of hibernation factors that protect ribosomes during dormancy and expand our understanding of ribosome hibernation in archaea.
リンク	bioRxiv / PubMed:41867869 / PubMed Central
手法	EM (単粒子)
解像度	1.94 - 2.96 Å
構造データ	74446 9znf EMDB-74446: Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB composite map PDB-9znf: Cryo-EM structure of the Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB 手法: EM (単粒子) / 解像度: 2.03 Å 74447 9zng EMDB-74447: Methanosarcina acetivorans 70S ribosome in complex with SriD composite map PDB-9zng: Cryo-EM structure of the Methanosarcina acetivorans 70S ribosome in complex with SriD 手法: EM (単粒子) / 解像度: 2.06 Å 74448 9zni EMDB-74448: Methanosarcina acetivorans 70S ribosome in complex with SriC and SriD composite map PDB-9zni: Cryo-EM structure of the Methanosarcina acetivorans 70S ribosome in complex with SriC and SriD 手法: EM (単粒子) / 解像度: 2.89 Å EMDB-74841: Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB consensus map 手法: EM (単粒子) / 解像度: 2.03 Å EMDB-74851: Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB 50S focused refinement 手法: EM (単粒子) / 解像度: 1.97 Å EMDB-74853: Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB 30S focused refinement 手法: EM (単粒子) / 解像度: 2.32 Å EMDB-74854: Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB 30S head focused refinement 手法: EM (単粒子) / 解像度: 2.48 Å EMDB-74982: Methanosarcina acetivorans 70S ribosome in complex with SriD consensus map 手法: EM (単粒子) / 解像度: 2.06 Å EMDB-74984: Methanosarcina acetivorans 70S ribosome in complex with SriD 50S focused refinement 手法: EM (単粒子) / 解像度: 1.94 Å EMDB-74985: Methanosarcina acetivorans 70S ribosome in complex with SriD 30S focused refinement 手法: EM (単粒子) / 解像度: 2.27 Å EMDB-74988: Methanosarcina acetivorans 70S ribosome in complex with SriC and SriD consensus map 手法: EM (単粒子) / 解像度: 2.89 Å EMDB-74989: Methanosarcina acetivorans 70S ribosome in complex with SriC and SriD 50S focused refinement 手法: EM (単粒子) / 解像度: 2.69 Å EMDB-74990: Methanosarcina acetivorans 70S ribosome in complex with SriC and SriD 30S focused refinement 手法: EM (単粒子) / 解像度: 2.92 Å EMDB-74992: Methanosarcina acetivorans 70S ribosome in complex with SriC and SriD 30S head focused refinement 手法: EM (単粒子) / 解像度: 2.96 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-HOH: WATER
由来	methanosarcina acetivorans (古細菌)
キーワード	RIBOSOME / Hibernation / Archaea