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Structure paper

TitleDNA-PK driven nucleosome unwrapping enables NHEJ in chromatin
Journal, issue, pagesNat Commun, Year 2026
Publish dateDec 10, 2025 (structure data deposition date)
AuthorsLu W / Vogt A / Lees-Miller SP / He Y
External linksNat Commun / Search PubMed
MethodsEM (single particle)
Resolution3.8 - 10.13 Å
Structure data

74422

9zmm

EMDB-74422, PDB-9zmm:
CryoEM structure of Ku heterodimer bound to N7 nucleosome
Method: EM (single particle) / Resolution: 3.89 Å

EMDB-74428: local refinement for N7
Method: EM (single particle) / Resolution: 3.89 Å

EMDB-74429: local reconstruction of Ku
Method: EM (single particle) / Resolution: 5.97 Å

EMDB-74432: Consensus map of Ku bound to N7 nucleosome
Method: EM (single particle) / Resolution: 9.42 Å

EMDB-74434: local reconstruction of DNA-PKcs
Method: EM (single particle) / Resolution: 3.93 Å

EMDB-74440: local reconstruction of Ku70/80
Method: EM (single particle) / Resolution: 4.05 Å

EMDB-74442: local reconstruction of N20 nucleosome
Method: EM (single particle) / Resolution: 6.19 Å

EMDB-74443: consensus map for DNA-PK bound to N20 nucleosome
Method: EM (single particle) / Resolution: 4.32 Å

74444

9znc

EMDB-74444, PDB-9znc:
CryoEM structure of the DNA-PK complex bound to N20 nucleosome
Method: EM (single particle) / Resolution: 3.93 Å

EMDB-74467: Local reconstruction of DNA-PK
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-74468: local reconstruction of N7
Method: EM (single particle) / Resolution: 7.24 Å

EMDB-74469: consensus map for DNA-PK bound to n7, state 2
Method: EM (single particle) / Resolution: 3.97 Å

74481

9zob

EMDB-74481, PDB-9zob:
CryoEM structure of DNA-PK bound to N7 nucleosome, state 2
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-74504: local reconstruction of DNA-PK
Method: EM (single particle) / Resolution: 4.11 Å

EMDB-74505: local reconstruction of N0 nucleosome
Method: EM (single particle) / Resolution: 10.13 Å

EMDB-74506: consensus map for DNA-PK bound to N0 nucleosome
Method: EM (single particle) / Resolution: 7.76 Å

74512

9zp0

EMDB-74512, PDB-9zp0:
CryoEM structure of DNA-PK bound to N0 nucleosome
Method: EM (single particle) / Resolution: 4.11 Å

EMDB-74519: consensus map for DNA-PK bound to N7 nucleosome, state 1
Method: EM (single particle) / Resolution: 3.88 Å

EMDB-74523: local reconstruction of N7 nucleosome
Method: EM (single particle) / Resolution: 8.38 Å

74524

9zp9

EMDB-74524, PDB-9zp9:
CryoEM structure of DNA-PK bound to N7 nucleosome, state 1
Method: EM (single particle) / Resolution: 3.88 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
  • xenopus laevis (African clawed frog)
KeywordsDNA BINDING PROTEIN/DNA / Complex / DNA repair / NHEJ / Nucleosome / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex / DNA-PK / Ku70/80

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