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Structure paper

TitleSubstrate selectivity of the human RNA m5C methyltransferase NSUN2
Journal, issue, pagesNature, Year 2026
Publish dateNov 5, 2025 (structure data deposition date)
AuthorsCanepa J / Ruiz-Arroyo VM / Schlamowitz N / Nam Y
External linksNature / Search PubMed
MethodsEM (single particle)
Resolution2.57 - 3.71 Å
Structure data

73772

9z2n

EMDB-73772, PDB-9z2n:
CryoEM structure of human NSUN2(C271A) with SAH cross-linked to tRNA Lys(CTT) (D-arm conformation)
Method: EM (single particle) / Resolution: 2.57 Å

73773

9z2o

EMDB-73773, PDB-9z2o:
CryoEM structure of human NSUN2(C271A) with SAH cross-linked to tRNA Lys(CTT) (No D-arm conformation)
Method: EM (single particle) / Resolution: 2.66 Å

73774

9z2p

EMDB-73774, PDB-9z2p:
CryoEM structure of human NSUN2 with tRNA Lys(CTT) (Conformation 1)
Method: EM (single particle) / Resolution: 2.91 Å

73775

9z2q

EMDB-73775, PDB-9z2q:
CryoEM structure of human NSUN2 with tRNA Lys(CTT) (Conformation 2)
Method: EM (single particle) / Resolution: 2.88 Å

73776

9z2r

EMDB-73776, PDB-9z2r:
CryoEM structure of human NSUN2 with tRNA Lys(CTT) and SFG (D-arm conformation)
Method: EM (single particle) / Resolution: 3.71 Å

73777

9z2t

EMDB-73777, PDB-9z2t:
CryoEM structure of human NSUN2 with tRNA Lys(CTT) and SFG (No D-arm conformation)
Method: EM (single particle) / Resolution: 3.7 Å

73778

9z2u

EMDB-73778, PDB-9z2u:
CryoEM structure of human NSUN2(C271A) with SAH cross-linked to tRNA Lys(TTT) (D-arm conformation)
Method: EM (single particle) / Resolution: 3.0 Å

73780

9z2w

EMDB-73780, PDB-9z2w:
CryoEM structure of human NSUN2(C271A) with SAH cross-linked to tRNA Lys(TTT) (No D-arm conformation)
Method: EM (single particle) / Resolution: 3.1 Å

73784

9z3d

EMDB-73784, PDB-9z3d:
CryoEM structure of human NSUN2(C271A) with SAH cross-linked to a pre-tRNA Leu(CAA) intron
Method: EM (single particle) / Resolution: 2.96 Å

Chemicals

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-SFG:
SINEFUNGIN

Source
  • homo sapiens (human)
KeywordsTRANSFERASE / Methyltransferase / tRNA / Complex

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