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TitleYeast Rad55-Rad57-SHU paralog complex dynamically promotes Rad51 filament formation.
Journal, issue, pagesMol Cell, Year 2026
Publish dateJul 21, 2026
AuthorsChristopher W Koo / Steven K Gore / Soo Y Ro / Jie Liu / Christine Yu / Caleigh M Azumaya / Bobby Brillantes / Inna Zilberleyb / Henry Chen / Mariam B Rafiqzada / Lyra Garcia Sanchez / Wolf-Dietrich Heyer / Claudio Ciferri / Stanislau Yatskevich /
PubMed AbstractHomologous recombination (HR) is an important DNA repair pathway that safeguards genome integrity. During HR, the Rad51 nucleoprotein filaments catalyze strand invasion into a homologous duplex DNA. ...Homologous recombination (HR) is an important DNA repair pathway that safeguards genome integrity. During HR, the Rad51 nucleoprotein filaments catalyze strand invasion into a homologous duplex DNA. Filament formation requires a conserved family of Rad51 paralogs that act as tumor suppressors in humans. By capturing six distinct states using cryo-electron microscopy, we reveal that the Saccharomyces cerevisiae Rad51 paralog complex, composed of the Rad55-Rad57 heterodimer and the SHU (Psy3-Csm2-Shu1-Shu2) complex, selectively brings Rad51 to single-stranded DNA to seed filament formation. Rad51 itself is a transient yet integral component of this machinery which binds along the Rad57 subunit to complete a high-affinity DNA-binding site. We also uncover a dual-nucleotide regulatory mechanism: a structural ADP molecule stabilizes the complex, while a second, catalytic ATPase site at the Rad57-Rad51 interface promotes the release of the paralog complex. These structural and mechanistic features provide a blueprint for understanding the function of Rad51 paralogs across eukaryotes.
External linksMol Cell / PubMed:42480524
MethodsEM (single particle)
Resolution2.74 - 5.75 Å
Structure data

EMDB-72245: Rad55-Rad57-SHU-Rad51-Rad51 bound to ssDNA with AMP-PNP. Local map focused on Rad55/Rad57
Method: EM (single particle) / Resolution: 3.07 Å

EMDB-72246: Rad55-Rad57-SHU-Rad51-Rad51 bound to ssDNA with AMP-PNP. Local map focused on SHU
Method: EM (single particle) / Resolution: 3.49 Å

EMDB-72247: Rad55-Rad57-SHU-Rad51-Rad51 bound to ssDNA with AMP-PNP. Local map focused on Rad51
Method: EM (single particle) / Resolution: 3.37 Å

EMDB-72249: Rad55-Rad57-SHU-Rad51-Rad51 bound to ssDNA with AMP-PNP. Composite map
Method: EM (single particle) / Resolution: 3.06 Å

EMDB-72252: Rad55-Rad57(E161Q)-SHU-Rad51-Rad51 bound to ssDNA with ATP. Local map focused on Rad55/Rad57
Method: EM (single particle) / Resolution: 3.37 Å

EMDB-72253: Rad55-Rad57(E161Q)-SHU-Rad51-Rad51 bound to ssDNA with ATP. Local map focused on SHU
Method: EM (single particle) / Resolution: 3.74 Å

EMDB-72254: Rad55-Rad57(E161Q)-SHU-Rad51-Rad51 bound to ssDNA with ATP. Local map focused on Rad51
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-72259: Rad55-Rad57(E161Q)-SHU-Rad51-Rad51 bound to ssDNA with ATP. Composite map
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-72261: Rad55-Rad57(E161Q)-SHU-3xRad51 bound to ssDNA with ATP. Local map focused on Rad55/Rad57
Method: EM (single particle) / Resolution: 3.72 Å

EMDB-72262: Rad55-Rad57(E161Q)-SHU-3xRad51 bound to ssDNA with ATP. Local map focused on SHU
Method: EM (single particle) / Resolution: 5.75 Å

EMDB-72263: Rad55-Rad57(E161Q)-SHU-3xRad51 bound to ssDNA with ATP. Local map focused on Rad51
Method: EM (single particle) / Resolution: 3.68 Å

EMDB-72264: Rad55-Rad57(E161Q)-SHU-3xRad51 bound to ssDNA with ATP. Composite map
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-72270: Rad55-Rad57-SHU homologous recombination complex. Composite map
Method: EM (single particle) / Resolution: 2.74 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)
  • synthetic construct (others)

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