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TitleIn situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS.
Journal, issue, pagesFEBS Lett, Year 2026
Publish dateJan 9, 2026
AuthorsPrzemysław Dutka / Yuxi Liu / Stefano Maggi / Debnath Ghosal / Jue Wang / Prashant P Damke / Stephen D Carter / Wei Zhao / Sukhithasri Vijayrajratnam / Joseph P Vogel / Carrie L Shaffer / Grant J Jensen /
PubMed AbstractThe Dot/Icm type IV secretion system (T4SS) is essential for Legionella pneumophila infection, but its in situ architecture and mechanism remain incompletely understood. Using cryo-electron ...The Dot/Icm type IV secretion system (T4SS) is essential for Legionella pneumophila infection, but its in situ architecture and mechanism remain incompletely understood. Using cryo-electron tomography, we performed subtomogram averaging and 3D classification to resolve structural heterogeneity within the complex. We identified multiple assembly states of the inner membrane complex, including a fully assembled form with a hexamer-of-dimers DotO ATPase and symmetry mismatches between subcomplexes. A composite in situ model revealed a central channel above the inner membrane, likely used for substrate secretion. Imaging of infected macrophages showed T4SSs tethered to host vacuoles and extracellular vesicle release, suggesting additional effector delivery routes. These findings provide insight into Dot/Icm T4SS structure and infection-related dynamics.
External linksFEBS Lett / PubMed:41510630
MethodsEM (subtomogram averaging)
Resolution21.0 - 30.0 Å
Structure data

EMDB-71739: Legionella Dot/Icm T4SS
Method: EM (subtomogram averaging) / Resolution: 30.0 Å

EMDB-72186: Focused refinement map of the periplasmic part of the Legionella pneumophila T4SS.
Method: EM (subtomogram averaging) / Resolution: 21.0 Å

EMDB-72187: Focused refinement map of the cytoplasmic region of the Legionella pneumophila T4SS.
Method: EM (subtomogram averaging) / Resolution: 30.0 Å

Source
  • Legionella pneumophila (bacteria)

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