| Title | Structures of RNase P ribozyme catalysis in apo and holo states |
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| Journal, issue, pages | To Be Published |
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| Publish date | Jun 2, 2025 (structure data deposition date) |
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Authors | Lee Y-T / Degenhardt MFS / Skeparnias I / Chen S-Y / Bhoge BA / Zhang J / Stagno JR / Wang Y-X |
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External links | Search PubMed |
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| Methods | EM (single particle) |
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| Resolution | 2.69 - 3.02 Å |
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| Structure data | EMDB-70934, PDB-9owk: Structure of Geobacillus stearothermophilus RNase P ribozyme in complex with precursor tRNA in 10 mM Ca2+ Method: EM (single particle) / Resolution: 2.98 Å EMDB-70938, PDB-9owo: Structure of Geobacillus stearothermophilus RNase P ribozyme in complex with precursor tRNA with non-complementary 5' leader (sub-conformation 1 of tRNA anticodon arm tilted) Method: EM (single particle) / Resolution: 2.93 Å EMDB-70939, PDB-9owp: Structure of Geobacillus stearothermophilus RNase P ribozyme in complex with precursor tRNA with non-complementary 5' leader (sub-conformation 2 of tRNA anticodon arm tilted) Method: EM (single particle) / Resolution: 2.94 Å EMDB-70942, PDB-9ows: Structure of Geobacillus stearothermophilus RNase P holoenzyme in 5 mM Mg2+ Method: EM (single particle) / Resolution: 2.87 Å EMDB-70943, PDB-9owt: Structure of Geobacillus stearothermophilus RNase P holoenzyme in complex with precursor tRNA in 5 mM Ca2+ Method: EM (single particle) / Resolution: 2.82 Å EMDB-70944, PDB-9owu: Structure of Geobacillus stearothermophilus RNase P holoenzyme in complex with precursor tRNA in 1 mM Ca2+ Method: EM (single particle) / Resolution: 2.93 Å EMDB-70945, PDB-9owv: Structure of Geobacillus stearothermophilus RNase P holoenzyme in complex with mature tRNA in 5 mM Ca2+ Method: EM (single particle) / Resolution: 3.02 Å EMDB-70946, PDB-9oww: Structure of Geobacillus stearothermophilus RNase P holoenzyme in complex with the precursor tRNA with non-complementary 5' leader Method: EM (single particle) / Resolution: 2.83 Å EMDB-70947, PDB-9owx: Structure of Geobacillus stearothermophilus RNase P holoenzyme in complex with the precursor tRNA with loop-back 5' leader (sub-conformation 1 of tRNA anticodon arm tilted) Method: EM (single particle) / Resolution: 2.7 Å EMDB-70948, PDB-9owy: Structure of Geobacillus stearothermophilus RNase P holoenzyme in complex with the precursor tRNA with loop-back 5' leader (sub-conformation 2 of tRNA anticodon arm tilted) Method: EM (single particle) / Resolution: 2.69 Å |
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| Chemicals | |
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| Source |  geobacillus stearothermophilus (bacteria)
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Keywords | RNA / ribozyme / RNase P / RNase P. / RNA/Hydrolase / RNA-Hydrolase complex |
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