Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Functional implications of the conformational landscape of a multidrug transporter revealed by Zebrafish Abcb4 structures.
Journal, issue, pages	Nat Commun, Year 2026
Publish date	May 30, 2026
Authors	Jingyu Zhan / Chao-Ming Hsieh / Lothar Esser / Zabrina C Lang / Abraham J Morton / Robert Robey / Fei Zhou / Suresh V Ambudkar / Rick K Huang / Michael M Gottesman / Di Xia /
PubMed Abstract	The hallmark of multidrug resistance conferred by the human ABC transporter ABCB1 (hP-gp) is the recognition and efflux of a diverse range of drugs, though the precise mechanism of polyspecificity ...The hallmark of multidrug resistance conferred by the human ABC transporter ABCB1 (hP-gp) is the recognition and efflux of a diverse range of drugs, though the precise mechanism of polyspecificity remains unresolved. In aquatic animals such as zebrafish, Abcb4, a functional homolog of hP-gp, plays a vital role in surviving environmental toxicants. Here, we show that DrAbcb4 exhibits comparable basal and drug-stimulated ATPase activity to hP-gp. Using cryo-EM, we capture five inward-facing DrAbcb4 conformations with varying separations between its two lobes, illustrating its open-and-close motion. The range of separation exceeds that seen in published P-gp structures that appear to be conformationally restricted. This global open-and-close motion is coupled with individual helix movement, resulting in a highly fluid substrate-binding pocket. These dynamic changes, likely underlying the polyspecificity of substrate recognition, predict unconventional protein-ligand interactions that are supported by structures of DrAbcb4 bound to the P-gp inhibitors tariquidar and elacridar, and the substrate vincristine.
External links	Nat Commun / PubMed:42218127
Methods	EM (single particle)
Resolution	3.0 - 4.09 Å
Structure data	70380 9ody EMDB-70380, PDB-9ody: Zebrafish Abcb4 in IF-narrow conformation (IF-Narrow) Method: EM (single particle) / Resolution: 3.29 Å 70381 9odz EMDB-70381, PDB-9odz: Zebrafish Abcb4 in IF-Wide conformation (IF-Wide) Method: EM (single particle) / Resolution: 3.59 Å 70382 9oe0 EMDB-70382, PDB-9oe0: Zebrafish Abcb4 in IF-Medium conformation (IF-Medium) Method: EM (single particle) / Resolution: 3.39 Å 70383 9oe1 EMDB-70383, PDB-9oe1: Zebrafish Abcb4 in IF-narrow conformation in the presence of Tariquidar (TQR-IF-Narrow) Method: EM (single particle) / Resolution: 3.02 Å 70384 9oe2 EMDB-70384, PDB-9oe2: Zebrafish Abcb4 in IF-medium conformation in the presence of Tariquidar (TQR-IF-Medium) Method: EM (single particle) / Resolution: 3.18 Å 70386 9oe4 EMDB-70386, PDB-9oe4: Zebrafish Abcb4 in IF-Narrow conformation in the presence of Elacridar (ELA-IF-Narrow) Method: EM (single particle) / Resolution: 3.0 Å 70387 9oe5 EMDB-70387, PDB-9oe5: Zebrafish Abcb4 in IF-Medium conformation in the presence of Elacridar (ELA-IF-Medium) Method: EM (single particle) / Resolution: 3.16 Å 70388 9oe6 EMDB-70388, PDB-9oe6: Zebrafish Abcb4 in IF-Wide conformation in the presence of Elacridar (ELA-IF-Wide) Method: EM (single particle) / Resolution: 3.31 Å 70389 9oe7 EMDB-70389, PDB-9oe7: Zebrafish Abcb4 in IF-narrow conformation in the presence of Vincristine (VCR-IF-Narrow) Method: EM (single particle) / Resolution: 3.95 Å 70390 9oe8 EMDB-70390, PDB-9oe8: Zebrafish Abcb4 in IF-medium conformation in the presence of Vincristine (VCR-IF-Medium) Method: EM (single particle) / Resolution: 3.7 Å 70391 9oe9 EMDB-70391, PDB-9oe9: Zebrafish Abcb4 in IF-Wide conformation in the presence of Vincristine (VCR-IF-Wide) Method: EM (single particle) / Resolution: 4.09 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogueYM ChemComp-HOH: WATER ChemComp-R1H: tariquidar ChemComp-CLR: CHOLESTEROL ChemComp-R0Z: elacridar ChemComp-CHD: CHOLIC ACID ChemComp-R1Q*: vincristine
Source	danio rerio (zebrafish)
Keywords	TRANSPORT PROTEIN / Multidrug resistance / Zebrafish Abcb4 / P-gp / Tariquidar / Elacridar / Vincristine