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TitleHeterogeneity of radial spoke components in Tetrahymena cilia.
Journal, issue, pagesCell Mol Life Sci, Vol. 82, Issue 1, Page 329, Year 2025
Publish dateAug 31, 2025
AuthorsMarta Bicka / Avrin Ghanaeian / Corbin Black / Ewa Joachimiak / Anna Osinka / Sumita Majhi / Anna Konopka / Ewa Bulska / Khanh Huy Bui / Dorota Wloga /
PubMed AbstractRadial spokes, RS1, RS2, and RS3, are T-shaped, multiprotein complexes that transmit regulatory signals from the central apparatus to outer doublet complexes, including dynein arms. Radial spokes, ...Radial spokes, RS1, RS2, and RS3, are T-shaped, multiprotein complexes that transmit regulatory signals from the central apparatus to outer doublet complexes, including dynein arms. Radial spokes, especially RS3, differ in their morphology, protein composition, and RS base-docked IDAs. Spokes' defects alter cilia beating frequency, waveform, and amplitude, leading, in humans, to primary ciliary dyskinesia and male infertility. The role of the particular spokes remains unclear. Ciliate Tetrahymena thermophila has three RSP3 paralogs and two or three paralogs of some other RSPs. Using multiple complementary approaches, we demonstrated that Tetrahymena forms RS1 and RS2 subtypes, each with a core composed of various RSP3 paralogs and one type of RSP3-less RS3. We elucidated proteomes of RS1 and RS2 subtypes, RS3, and identified novel RS proteins, including enzymatic proteins involved in local regulation of ADP/ATP levels, the guanylate nucleotide pathway, and protein phosphorylation, whose presence further diversifies RSs' properties and likely functions.
External linksCell Mol Life Sci / PubMed:40886204 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution17.16 - 33.13 Å
Structure data

EMDB-70169: Subtomogram Averaged Cryo-ET Structure of the 96-nm Axonemal Repeat in RSP3A-KO Tetrahymena thermophila
Method: EM (subtomogram averaging) / Resolution: 22.26 Å

EMDB-70171: Subtomogram Averaged Cryo-ET Structure of the 96-nm Axonemal Repeat in RSP3C-KO Tetrahymena thermophila
Method: EM (subtomogram averaging) / Resolution: 17.16 Å

EMDB-70172: Subtomogram Averaged Cryo-ET Structure of the 96-nm Axonemal Repeat in RSP3A-HA-BCCP Tetrahymena thermophila
Method: EM (subtomogram averaging) / Resolution: 33.13 Å

Source
  • Tetrahymena thermophila (eukaryote)

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