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-Structure paper
Title | Structures of the fully assembled spliceosome before activation. |
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Journal, issue, pages | Science, Vol. 360, Issue 6396, Page 1423-1429, Year 2018 |
Publish date | Jun 29, 2018 |
Authors | Rui Bai / Ruixue Wan / Chuangye Yan / Jianlin Lei / Yigong Shi / |
PubMed Abstract | The precatalytic spliceosome (B complex) is preceded by the pre-B complex. Here we report the cryo-electron microscopy structures of the pre-B and B complexes at average resolutions of 3.3 to 4.6 ...The precatalytic spliceosome (B complex) is preceded by the pre-B complex. Here we report the cryo-electron microscopy structures of the pre-B and B complexes at average resolutions of 3.3 to 4.6 and 3.9 angstroms, respectively. In the pre-B complex, the duplex between the 5' splice site (5'SS) and U1 small nuclear RNA (snRNA) is recognized by Yhc1, Luc7, and the Sm ring. In the B complex, U1 small nuclear ribonucleoprotein is dissociated, the 5'-exon-5'SS sequences are translocated near U6 snRNA, and three B-specific proteins may orient the precursor messenger RNA. In both complexes, U6 snRNA is anchored to loop I of U5 snRNA, and the duplex between the branch point sequence and U2 snRNA is recognized by the SF3b complex. Structural analysis reveals the mechanism of assembly and activation for the yeast spliceosome. |
External links | Science / PubMed:29794219 |
Methods | EM (single particle) |
Resolution | 3.4 - 3.9 Å |
Structure data | EMDB-6972, PDB-5zwm: |
Chemicals | ChemComp-GTP: ChemComp-MG: ChemComp-ZN: |
Source |
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Keywords | SPLICING / spliceosome / assembly / pre-B complex / U1 snRNP / spliceosme / assemply / B complex |