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TitleCryo-EM structures of the SurA-BAM complex reveal conformational changes in outer membrane protein assembly.
Journal, issue, pagesNat Commun, Vol. 17, Issue 1, Year 2026
Publish dateSep 4, 2026
AuthorsRyoji Miyazaki / Hidetaka Kohga / Nami Matsuoka / Yuki Maruno / Wataru Yoshimoto / Yutaro S Takahashi / Dede Heri Yuli Yanto / Yudhi Nugraha / Hideki Shigematsu / Takuya Shiota / Tomoya Tsukazaki /
PubMed AbstractThe outer membrane (OM) of Gram-negative bacteria acts as a permeability barrier against toxic compounds. Its integrity is maintained by various outer membrane proteins (OMPs), which are inserted ...The outer membrane (OM) of Gram-negative bacteria acts as a permeability barrier against toxic compounds. Its integrity is maintained by various outer membrane proteins (OMPs), which are inserted into the OM by the β-barrel assembly machinery (BAM) complex. The periplasmic chaperone SurA delivers unfolded OMPs to BAM; however, the mechanism of substrate transfer remains unclear. Here, we show that the flexible P1 and P2 domains of SurA regulate the function of its Core domain and interact with BAM components, including BamE, whose interaction with the P2 domain is crucial for efficient OMP assembly. Moreover, cryo-electron microscopy reveals four distinct Escherichia coli SurA-BAM structures, suggesting dynamic domain rearrangements of SurA. Based on these findings, we propose a dynamic model in which SurA transfers substrates to BAM through multiple conformational changes, providing a unified framework for chaperone-assisted OMP biogenesis.
External linksNat Commun / PubMed:42697891 / PubMed Central
MethodsEM (single particle)
Resolution3.04 - 4.2 Å
Structure data

66714

9xby

EMDB-66714, PDB-9xby:
BAM-SurA complex (P1-visible)
Method: EM (single particle) / Resolution: 3.9 Å

66821

9xfg

EMDB-66821, PDB-9xfg:
BAM-SurA complex (P2-visible 2)
Method: EM (single particle) / Resolution: 3.7 Å

66834

9xfo

EMDB-66834, PDB-9xfo:
BAM-SurA complex (P2-visible 1)
Method: EM (single particle) / Resolution: 3.04 Å

69488

24gl

EMDB-69488, PDB-24gl:
BAM-SurA complex (P1_P2-visible 1)
Method: EM (single particle) / Resolution: 3.4 Å

69496

24gt

EMDB-69496, PDB-24gt:
BAM-SurA complex (P1_P2-visible 2)
Method: EM (single particle) / Resolution: 4.2 Å

80076

25fq

EMDB-80076, PDB-25fq:
BAM-SurA complex (Core only)
Method: EM (single particle) / Resolution: 3.6 Å

Source
  • escherichia coli (E. coli)
KeywordsPROTEIN TRANSPORT / insertase / outer membrane protein / chaperone / protein folding / protein complex / membrane protein / Periplasmic chaperon

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