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Structure paper

TitleDNA polymerization activates RNA cleavage of a reverse transcriptase-like antiviral enzyme
Journal, issue, pagesScience, Year 2026
Publish dateDec 22, 2025 (structure data deposition date)
AuthorsRong X / Xiao J / Zhao X / Yan Y / Li J / Chen Y / Fan Y / Liu Z / Cao Y / Chen F ...Rong X / Xiao J / Zhao X / Yan Y / Li J / Chen Y / Fan Y / Liu Z / Cao Y / Chen F / Cheng R / Wang X / Wang L / Zhu B
External linksScience / Search PubMed
MethodsEM (single particle)
Resolution2.22 - 2.96 Å
Structure data

67871

21ou

EMDB-67871, PDB-21ou:
DRT4 homohexamer
Method: EM (single particle) / Resolution: 2.34 Å

67946

21ro

EMDB-67946, PDB-21ro:
DRT4 homohexamer with dGTPaS
Method: EM (single particle) / Resolution: 2.68 Å

67947

21rp

EMDB-67947, PDB-21rp:
DRT4 homohexamer with dATP
Method: EM (single particle) / Resolution: 2.84 Å

67950

21rs

EMDB-67950, PDB-21rs:
DRT4 homohexamer with dATP, dGTPaS, SSB, RNA
Method: EM (single particle) / Resolution: 2.76 Å

68056

21wo

EMDB-68056, PDB-21wo:
DRT4 homohexamer with dATP, SSB
Method: EM (single particle) / Resolution: 2.96 Å

69681

24na

EMDB-69681, PDB-24na:
DRT4 homohexamer with dGTP
Method: EM (single particle) / Resolution: 2.8 Å

69682

24nb

EMDB-69682, PDB-24nb:
DRT4 homohexamer with dGTP, GMP
Method: EM (single particle) / Resolution: 2.54 Å

69683

24nc

EMDB-69683, PDB-24nc:
DRT4 homohexamer with dGTP, RNA
Method: EM (single particle) / Resolution: 2.22 Å

69684

24nd

EMDB-69684, PDB-24nd:
DRT4 homohexamer with dGTP, ssRNA (local refinement)
Method: EM (single particle) / Resolution: 2.68 Å

Chemicals

ChemComp-T8T:
2'-deoxyguanosine-5'-O-(1-thiotriphosphate)

ChemComp-MG:
Unknown entry

ChemComp-DTP:
2'-DEOXYADENOSINE 5'-TRIPHOSPHATE

ChemComp-GMP:
GUANOSINE

ChemComp-DGT:
2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE

ChemComp-DGP:
2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE

Source
  • enterobacteriaceae (enterobacteria)
  • escherichia coli (E. coli)
  • dna molecule (others)
  • synthetic construct (others)
KeywordsRNA BINDING PROTEIN/DNA / DRT4; reverse transcriptase; terminal transferase; nuclease / RNA BINDING PROTEIN-DNA complex / RNA BINDING PROTEIN / IMMUNE SYSTEM

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