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TitleCryo-EM structures of a MexB-MexY chimeric efflux pump reveal that large open clefts are intrinsic to the MexY porter domain.
Journal, issue, pagesActa Crystallogr F Struct Biol Commun, Vol. 82, Issue Pt 3, Page 83-93, Year 2026
Publish dateMar 1, 2026
AuthorsJiye Wang / Kenta Tsutsumi / Mika Hirose / Ryosuke Nakashima / Takayuki Kato / Kunihiko Nishino / Atsushi Nakagawa / Eiki Yamashita /
PubMed AbstractRND-type multidrug-efflux pumps are major contributors to multidrug resistance in Gram-negative bacteria, with MexY from Pseudomonas aeruginosa playing a central role in aminoglycoside resistance. ...RND-type multidrug-efflux pumps are major contributors to multidrug resistance in Gram-negative bacteria, with MexY from Pseudomonas aeruginosa playing a central role in aminoglycoside resistance. Unlike other RND transporters, MexY exhibits unusually large open clefts in the binding and extrusion states. To determine whether this feature is intrinsic to its drug-recognition porter domain, we created a chimeric protein, MexBYB, by replacing the funnel-like and transmembrane domains of MexY with those of the homologous transporter MexB, and determined its structures by cryoEM under apo and kanamycin-supplemented conditions. Under both conditions, MexBYB was reported to adopt symmetric-like and asymmetric conformations. Structural comparisons reveal that the unusually large open clefts are retained in MexBYB, indicating that this feature is intrinsic to the MexY porter domain.
External linksActa Crystallogr F Struct Biol Commun / PubMed:41744473 / PubMed Central
MethodsEM (single particle)
Resolution3.55 - 5.82 Å
Structure data

67812

22xk

EMDB-67812, PDB-22xk:
MexBYB-Ka asymmetry
Method: EM (single particle) / Resolution: 3.6 Å

67813

22xm

EMDB-67813, PDB-22xm:
MexBYB-Ka symmetry-like
Method: EM (single particle) / Resolution: 3.55 Å

EMDB-67814: MexBYB-apo asymmetry
Method: EM (single particle) / Resolution: 4.72 Å

EMDB-67815: MexBYB-apo symmetry-like
Method: EM (single particle) / Resolution: 5.82 Å

Source
  • pseudomonas aeruginosa (bacteria)
KeywordsMEMBRANE PROTEIN / RND / Chimera / Multidrug Efflux Pump

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