EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of an endogenous yeast 26S proteasome reveals two major conformational states.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 113, Issue 10, Page 2642-2647, Year 2016
掲載日	2016年3月8日
著者	Bai Luan / Xiuliang Huang / Jianping Wu / Ziqing Mei / Yiwei Wang / Xiaobin Xue / Chuangye Yan / Jiawei Wang / Daniel J Finley / Yigong Shi / Feng Wang /
PubMed 要旨	The eukaryotic proteasome mediates degradation of polyubiquitinated proteins. Here we report the single-particle cryoelectron microscopy (cryo-EM) structures of the endogenous 26S proteasome from ...The eukaryotic proteasome mediates degradation of polyubiquitinated proteins. Here we report the single-particle cryoelectron microscopy (cryo-EM) structures of the endogenous 26S proteasome from Saccharomyces cerevisiae at 4.6- to 6.3-Å resolution. The fine features of the cryo-EM maps allow modeling of 18 subunits in the regulatory particle and 28 in the core particle. The proteasome exhibits two distinct conformational states, designated M1 and M2, which correspond to those reported previously for the proteasome purified in the presence of ATP-γS and ATP, respectively. These conformations also correspond to those of the proteasome in the presence and absence of exogenous substrate. Structure-guided biochemical analysis reveals enhanced deubiquitylating enzyme activity of Rpn11 upon assembly of the lid. Our structures serve as a molecular basis for mechanistic understanding of proteasome function.
リンク	Proc Natl Acad Sci U S A / PubMed:26929360 / PubMed Central
手法	EM (単粒子)
解像度	4.6 - 8.7 Å
構造データ	6574 3jco EMDB-6574: Cryo-EM map of yeast 26S proteasome in M1 state derived from Titan dataset PDB-3jco: Structure of yeast 26S proteasome in M1 state derived from Titan dataset 手法: EM (単粒子) / 解像度: 4.8 Å 6575 3jcp EMDB-6575: Cryo-EM map of yeast 26S proteasome in M2 state derived from Titan dataset PDB-3jcp: Structure of yeast 26S proteasome in M2 state derived from Titan dataset 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-6576: Cryo-EM map of yeast 26S proteasome in M1 state derived from Arctica dataset 手法: EM (単粒子) / 解像度: 5.8 Å EMDB-6577: Cryo-EM map of yeast 26S proteasome in M2 state derived from Arctica dataset 手法: EM (単粒子) / 解像度: 8.3 Å EMDB-6578: Cryo-EM map of yeast 26S proteasome in M1 state with RP mask derived from Arctica dataset 手法: EM (単粒子) / 解像度: 6.3 Å EMDB-6579: Cryo-EM map of yeast 26S proteasome in M2 state with RP mask derived from Arctica dataset 手法: EM (単粒子) / 解像度: 8.7 Å
由来	Saccharomyces cerevisiae (パン酵母) saccharomyces cerevisiae s288c (パン酵母)
キーワード	HYDROLASE / protein complex