Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM Structures of the Magnesium Channel CorA Reveal Symmetry Break upon Gating.
Journal, issue, pages	Cell, Vol. 164, Issue 4, Page 747-756, Year 2016
Publish date	Feb 11, 2016
Authors	Doreen Matthies / Olivier Dalmas / Mario J Borgnia / Pawel K Dominik / Alan Merk / Prashant Rao / Bharat G Reddy / Shahidul Islam / Alberto Bartesaghi / Eduardo Perozo / Sriram Subramaniam /
PubMed Abstract	CorA, the major Mg(2+) uptake system in prokaryotes, is gated by intracellular Mg(2+) (KD ∼ 1-2 mM). X-ray crystallographic studies of CorA show similar conformations under Mg(2+)-bound and Mg(2+)- ...CorA, the major Mg(2+) uptake system in prokaryotes, is gated by intracellular Mg(2+) (KD ∼ 1-2 mM). X-ray crystallographic studies of CorA show similar conformations under Mg(2+)-bound and Mg(2+)-free conditions, but EPR spectroscopic studies reveal large Mg(2+)-driven quaternary conformational changes. Here, we determined cryo-EM structures of CorA in the Mg(2+)-bound closed conformation and in two open Mg(2+)-free states at resolutions of 3.8, 7.1, and 7.1 Å, respectively. In the absence of bound Mg(2+), four of the five subunits are displaced to variable extents (∼ 10-25 Å) by hinge-like motions as large as ∼ 35° at the stalk helix. The transition between a single 5-fold symmetric closed state and an ensemble of low Mg(2+), open, asymmetric conformational states is, thus, the key structural signature of CorA gating. This mechanism is likely to apply to other structurally similar divalent ion channels.
External links	Cell / PubMed:26871634 / PubMed Central
Methods	EM (single particle)
Resolution	3.8 - 7.06 Å
Structure data	6551 3jcf EMDB-6551, PDB-3jcf: Cryo-EM structure of the magnesium channel CorA in the closed symmetric magnesium-bound state Method: EM (single particle) / Resolution: 3.8 Å 6552 3jcg EMDB-6552: Cryo-EM structure of the magnesium channel CorA in the magnesium-free asymmetric open state I PDB-3jcg: Cryo-EM structure of the magnesium channel CorA in the magnesium-free, asymmetric open state I Method: EM (single particle) / Resolution: 7.06 Å 6553 3jch EMDB-6553: Cryo-EM structure of the magnesium channel CorA in the magnesium-free asymmetric open state II PDB-3jch: Cryo-EM structure of the magnesium channel CorA in the magnesium-free, asymmetric open state II Method: EM (single particle) / Resolution: 7.06 Å
Chemicals	ChemComp-MG: Unknown entry
Source	thermotoga maritima (bacteria)
Keywords	TRANSPORT PROTEIN / membrane protein / ion channel / magnesium channel / pentameric complex / symmetry vs. asymmetry / conformational change / gating mechanism / direct electron detector / K2