EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Translation landscape of stress granules.
ジャーナル・号・ページ	Sci Adv, Vol. 11, Issue 40, Page eady6859, Year 2025
掲載日	2025年10月3日
著者	Yichun Wu / Xing Wang / Lingyu Meng / Zhizhao Liao / Wei Ji / Peipei Zhang / Jie Lin / Qiang Guo /
PubMed 要旨	Stress granules, cytoplasmic assemblies of RNA binding proteins and messenger RNAs formed during cellular stress, are implicated in translational control. However, their exact functions remain ...Stress granules, cytoplasmic assemblies of RNA binding proteins and messenger RNAs formed during cellular stress, are implicated in translational control. However, their exact functions remain elusive. Here, we used cryogenic correlative light and electron microscopy to visualize stress granules in their native environment and reconstructed them in three dimensions using tomography. This approach provided the first quantitative and spatial analysis of the translational machinery within stress granules. Our findings suggest that stress granules have a limited impact on global translation regulation but serve to protect small ribosomal subunits and preinitiation complexes from degradation. Numerical simulations based on a phase-field model accurately reproduced the spatial distribution of ribosomal components inside and outside the stress granules, shedding light on the thermodynamic principles governing this process.
リンク	Sci Adv / PubMed:41042876 / PubMed Central
手法	EM (サブトモグラム平均)
解像度	6.3 - 19.5 Å
構造データ	EMDB-65457: Pre-initiation complex class 1 with eIF4A of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 16.1 Å EMDB-65458: 80S ribosome of HeLa cells treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 6.3 Å EMDB-65459: 80S ribosome class 1 with eEF1A,A/T,P in unrotated state of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 15.5 Å EMDB-65460: 80S ribosome class 2 with eEF2 in unrotated state of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 9.9 Å EMDB-65461: Pre-initiation complex class 2 with eIF4A and eIF4G of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 18.9 Å EMDB-65462: 80S ribosome class 3 with eEF2,Z-tRNA in unrotated state of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 19.5 Å EMDB-65463: Pre-initiation complex of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 10.0 Å EMDB-65464: 80S ribosome class 4 with eEF1A,A/T in unrotated state of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 9.9 Å EMDB-65465: 80S ribosome class 5 with E-tRNA in unrotated state of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 18.2 Å EMDB-65466: 80S ribosome class 6 with eEF1A,A/T,E in unrotated state of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 10.9 Å EMDB-65467: 80S ribosome class 7 with eEF2,eIF5A in rotated state of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 8.1 Å EMDB-65468: 80S ribosome class 8 with eEF2,Z-tRNA in rotated state of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 13.3 Å EMDB-65469: 80S ribosome class 9 with eEF2,E-tRNA in rotated state of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 9.2 Å EMDB-65470: 80S ribosome class 10 with eEF2 in rotated state of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 8.6 Å EMDB-65471: 40S ribosome subunit of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 11.0 Å EMDB-65472: 40S ribosome subunit class 1 with eIF1A of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 15.2 Å EMDB-65473: 40S ribosome subunit class 2 with eIF1A,eIF1 of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 15.2 Å EMDB-65474: Pre-initiation complex class 3 of HeLa cell treated by sodium arsenite 手法: EM (サブトモグラム平均) / 解像度: 11.4 Å
由来	Homo sapiens (ヒト)