Movie
Controller
Structure viewers
About Yorodumi Papers
+Search query
-Structure paper
| Title | Translation landscape of stress granules. |
|---|---|
| Journal, issue, pages | Sci Adv, Vol. 11, Issue 40, Page eady6859, Year 2025 |
| Publish date | Oct 3, 2025 |
 Authors | Yichun Wu / Xing Wang / Lingyu Meng / Zhizhao Liao / Wei Ji / Peipei Zhang / Jie Lin / Qiang Guo /  |
| PubMed Abstract | Stress granules, cytoplasmic assemblies of RNA binding proteins and messenger RNAs formed during cellular stress, are implicated in translational control. However, their exact functions remain ...Stress granules, cytoplasmic assemblies of RNA binding proteins and messenger RNAs formed during cellular stress, are implicated in translational control. However, their exact functions remain elusive. Here, we used cryogenic correlative light and electron microscopy to visualize stress granules in their native environment and reconstructed them in three dimensions using tomography. This approach provided the first quantitative and spatial analysis of the translational machinery within stress granules. Our findings suggest that stress granules have a limited impact on global translation regulation but serve to protect small ribosomal subunits and preinitiation complexes from degradation. Numerical simulations based on a phase-field model accurately reproduced the spatial distribution of ribosomal components inside and outside the stress granules, shedding light on the thermodynamic principles governing this process. |
 External links | Sci Adv / PubMed:41042876 / PubMed Central |
| Methods | EM (subtomogram averaging) |
| Resolution | 6.3 - 19.5 Å |
| Structure data |  EMDB-65457: Pre-initiation complex class 1 with eIF4A of HeLa cell treated by sodium arsenite  EMDB-65458: 80S ribosome of HeLa cells treated by sodium arsenite  EMDB-65459: 80S ribosome class 1 with eEF1A,A/T,P in unrotated state of HeLa cell treated by sodium arsenite  EMDB-65460: 80S ribosome class 2 with eEF2 in unrotated state of HeLa cell treated by sodium arsenite  EMDB-65461: Pre-initiation complex class 2 with eIF4A and eIF4G of HeLa cell treated by sodium arsenite  EMDB-65462: 80S ribosome class 3 with eEF2,Z-tRNA in unrotated state of HeLa cell treated by sodium arsenite  EMDB-65463: Pre-initiation complex of HeLa cell treated by sodium arsenite  EMDB-65464: 80S ribosome class 4 with eEF1A,A/T in unrotated state of HeLa cell treated by sodium arsenite  EMDB-65465: 80S ribosome class 5 with E-tRNA in unrotated state of HeLa cell treated by sodium arsenite  EMDB-65466: 80S ribosome class 6 with eEF1A,A/T,E in unrotated state of HeLa cell treated by sodium arsenite  EMDB-65467: 80S ribosome class 7 with eEF2,eIF5A in rotated state of HeLa cell treated by sodium arsenite  EMDB-65468: 80S ribosome class 8 with eEF2,Z-tRNA in rotated state of HeLa cell treated by sodium arsenite  EMDB-65469: 80S ribosome class 9 with eEF2,E-tRNA in rotated state of HeLa cell treated by sodium arsenite  EMDB-65470: 80S ribosome class 10 with eEF2 in rotated state of HeLa cell treated by sodium arsenite  EMDB-65471: 40S ribosome subunit of HeLa cell treated by sodium arsenite  EMDB-65472: 40S ribosome subunit class 1 with eIF1A of HeLa cell treated by sodium arsenite  EMDB-65473: 40S ribosome subunit class 2 with eIF1A,eIF1 of HeLa cell treated by sodium arsenite  EMDB-65474: Pre-initiation complex class 3 of HeLa cell treated by sodium arsenite |
| Source |
|
Homo sapiens (human)