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TitleStructure of Bacillus subtilis Ku-mediated DNA synaptic complex.
Journal, issue, pagesNucleic Acids Res, Vol. 54, Issue 1, Year 2026
Publish dateJan 5, 2026
AuthorsWhan-Jong Kim / Jieun Kim / Mingyu Jo / Youngjin Kim / Min-Sung Kim /
PubMed AbstractDNA double-strand breaks (DSBs) pose a severe threat to genomic integrity, and cells rely on two major pathways for repair: homologous recombination and non-homologous end joining (NHEJ). While ...DNA double-strand breaks (DSBs) pose a severe threat to genomic integrity, and cells rely on two major pathways for repair: homologous recombination and non-homologous end joining (NHEJ). While eukaryotic NHEJ requires a multi-component assembly including the Ku70/80 heterodimer, bacterial NHEJ operates with a simpler toolkit comprising a Ku homodimer and the multifunctional LigD. Despite this simplicity, the mechanism by which broken DNA ends are bridged together has remained unclear in bacterial NHEJ. Here, we present a cryo-electron microscopy structure of the Bacillus subtilis Ku (bsKu)-DNA complex at 2.74 Å resolution, capturing two blunt DNA ends bridged by a Ku protein alone. Supported by further biochemical assays, we propose an integrated model in which oligomeric arrays of Ku homodimers bridge and stabilize two DNA ends, facilitating efficient DSB repair in Bacillus subtilis. This work reveals a bsKu-mediated DNA bridging mechanism distinct from the eukaryotic system and provides critical structural insight into prokaryotic DNA repair.
External linksNucleic Acids Res / PubMed:41118517 / PubMed Central
MethodsEM (single particle)
Resolution2.74 Å
Structure data

65215

9vnq

EMDB-65215, PDB-9vnq:
Bacillus Subtilis Ku core homodimer complexed with double strand DNA
Method: EM (single particle) / Resolution: 2.74 Å

Source
  • bacillus subtilis (bacteria)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN / Non homologous end joining / DNA bridging

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