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TitleThe influence of frame alignment with dose compensation on the quality of single particle reconstructions.
Journal, issue, pagesJ Struct Biol, Vol. 192, Issue 2, Page 196-203, Year 2015
Publish dateSep 25, 2015
AuthorsJohn M Spear / Alex J Noble / Qing Xie / Duncan R Sousa / Michael S Chapman / Scott M Stagg /
PubMed AbstractAs direct electron detection devices in cryo-electron microscopy become ubiquitous, the field is now ripe for new developments in image analysis techniques that take advantage of their increased SNR ...As direct electron detection devices in cryo-electron microscopy become ubiquitous, the field is now ripe for new developments in image analysis techniques that take advantage of their increased SNR coupled with their high-throughput frame collection abilities. In approaching atomic resolution of native-like biomolecules, the accurate extraction of structural locations and orientations of side-chains from frames depends not only on the electron dose that a sample receives but also on the ability to accurately estimate the CTF. Here we use a new 2.8Å resolution structure of a recombinant gene therapy virus, AAV-DJ with Arixtra, imaged on an FEI Titan Krios with a DE-20 direct electron detector to probe new metrics including relative side-chain density and ResLog analysis for optimizing the compensation of electron beam damage and to characterize the factors that are limiting the resolution of the reconstruction. The influence of dose compensation on the accuracy of CTF estimation and particle classifiability are also presented. We show that rigorous dose compensation allows for better particle classifiability and greater recovery of structural information from negatively charged, electron-sensitive side-chains, resulting in a more accurate macromolecular model.
External linksJ Struct Biol / PubMed:26391007 / PubMed Central
MethodsEM (single particle)
Resolution2.8 Å
Structure data

EMDB-6470:
Single particle reconstruction of AAV-DJ in complex with ARIXTRA
Method: EM (single particle) / Resolution: 2.8 Å

Source
  • unidentified (others)

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