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Structure paper

TitleA Rapid and Universal Pipeline for High-Resolution GPCR Structure Determination through In Silico Construct Optimization and de novo Protein Design
Journal, issue, pagesBiorxiv, Year 2026
Publish dateMay 11, 2025 (structure data deposition date)
AuthorsKojima A / Kawakami K / Kobayashi N / Kobayashi K / Matsui TE / Uemoto K / Gu Y / Fukuda M / Kato HE
External linksBiorxiv / Search PubMed
MethodsEM (single particle)
Resolution2.89 - 3.69 Å
Structure data

EMDB-64536, PDB-9uvt:
Cryo-EM structure of bradykinin B2 receptor (B2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with icatibant
Method: EM (single particle) / Resolution: 3.29 Å

EMDB-64537, PDB-9uvu:
Cryo-EM structure of bradykinin B2 receptor (B2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with icatibant, focused on receptor
Method: EM (single particle) / Resolution: 3.69 Å

EMDB-64538, PDB-9uvv:
Cryo-EM structure of Vasopressin receptor 2 (V2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with tolvaptan
Method: EM (single particle) / Resolution: 3.02 Å

EMDB-64539, PDB-9uvw:
Cryo-EM structure of Vasopressin receptor 2 (V2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with tolvaptan, focused on receptor
Method: EM (single particle) / Resolution: 2.89 Å

EMDB-64540, PDB-9uvx:
Cryo-EM structure of Vasopressin receptor 2 (V2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with OPC51803, focused on receptor
Method: EM (single particle) / Resolution: 3.31 Å

EMDB-64541, PDB-9uvy:
Cryo-EM structure of Vasopressin receptor 2 (V2R)-ARK1 with tolvaptan
Method: EM (single particle) / Resolution: 2.98 Å

EMDB-64542, PDB-9uvz:
Cryo-EM structure of Lysophosphatidic acid receptor 2 (LPA2)-ARK1 with Ki16425
Method: EM (single particle) / Resolution: 2.99 Å

Chemicals

PDB-1it8:
Crystal structure of archaeosine tRNA-guanine transglycosylase from Pyrococcus horikoshii complexed with archaeosine precursor, preQ0

PDB-1l9o:
CRYSTAL STRUCTURE OF NITRITE SOAKED I257A VARIANT OF THE COPPER-CONTAINING NITRITE REDUCTASE FROM ALCALIGENES FAECALIS

ChemComp-HOH:
WATER

PDB-1l9p:
CRYSTAL STRUCTURE OF NITRITE SOAKED I257G VARIANT OF THE COPPER-CONTAINING NITRITE REDUCTASE FROM ALCALIGENES FAECALIES S-6

ChemComp-NA:
Unknown entry

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
  • synthetic construct (others)
  • escherichia coli (E. coli)
KeywordsMEMBRANE PROTEIN / GPCR / B2R / bradykinin / icatibant / V2R / vasopressin / tolvaptan / OPC51803 / ARK1 / LPA2 / Lysophosphatidic acid receptor 2 / Ki16425

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