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TitleEnhancing the synthesis efficiency of galacto-oligosaccharides of a β-galactosidase from Paenibacillus barengoltzii by engineering the active and distal sites.
Journal, issue, pagesFood Chem, Vol. 483, Page 144208, Year 2025
Publish dateApr 4, 2025
AuthorsHaiyan Yu / Yulu Wang / Zhisen Yang / Jiabao Ying / Feifei Guan / Bolin Liu / Miao Miao / Abeer Mohamed / Xue Wei / Yuji Yang / Xin Liu / Linfeng Sun / Zhengqiang Jiang / Shaoqing Yang / Fengjiao Xin /
PubMed AbstractPreviously, a glycoside hydrolase (GH) family 2 β-galactosidase (PbBGal2A) from Paenibacillus barengoltzii is characterized for its high transglycosylation capability. Here, the cryo-electron ...Previously, a glycoside hydrolase (GH) family 2 β-galactosidase (PbBGal2A) from Paenibacillus barengoltzii is characterized for its high transglycosylation capability. Here, the cryo-electron microscopy (cryo-EM) structure of PbBGal2A was determined, revealing an enlarged acidic catalytic pocket that facilitate the binding of carbohydrate substrates. Three structure-based strategies as well as machine learning MECE platform (method for enhancing the catalytic efficiency) were employed to identify active and distal mutations with enhanced galacto-oligosaccharides (GOS) synthesis and their synergistic effects were evaluated. The best H331V mutation yielded a maximum GOS production of 76.57 % at 4 h when 35 % (w/v) of lactose was used as a substrate. Molecular dynamics (MD) simulation analysis further indicated that distal mutations increase the rigidity of the loops surrounding the catalytic pocket. This research sheds light on the structural and catalytic mechanisms of PbBGal2A, highlighting the importance of both active and distal mutations in the efficient design of customized β-galactosidases.
External linksFood Chem / PubMed:40220440
MethodsEM (single particle)
Resolution2.8 Å
Structure data

63544

9m0e

EMDB-63544, PDB-9m0e:
Enhancing the synthesis efficiency of galacto-oligosaccharides of a beta-galactosidase from Paenibacillus barengoltzii by engineering the active and distal sites
Method: EM (single particle) / Resolution: 2.8 Å

Source
  • paenibacillus barengoltzii (bacteria)
KeywordsHYDROLASE / Beta-galactosidase / transglycosylation / GOS synthesis / cryo-EM structure

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