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TitleDe novo designed voltage-gated anion channels suppress neuron firing.
Journal, issue, pagesCell, Vol. 188, Issue 26, Page 7495-7511.e21, Year 2025
Publish dateDec 24, 2025
AuthorsChen Zhou / Huican Li / Jiaxing Wang / Cheng Qian / Hui Xiong / Zhilin Chu / Qiming Shao / Xuan Li / Shijin Sun / Ke Sun / Aiqin Zhu / Jiawei Wang / Xueqin Jin / Fan Yang / Tamer M Gamal El-Din / Bo Li / Jing Huang / Kun Wu / Peilong Lu /
PubMed AbstractDesign of ion channels responsive to environmental cues has significant implications in modulating cellular activities and sensor development, but it remains a significant challenge due to the ...Design of ion channels responsive to environmental cues has significant implications in modulating cellular activities and sensor development, but it remains a significant challenge due to the complexities involved in designing stimuli-induced conformational changes in proteins. Here, we report the accurate de novo design of voltage-gated anion channels, namely dVGACs. dVGACs adopt a 15-helix pentameric architecture featuring arginine constrictions within the transmembrane span and show voltage-dependent anions currents in patch-clamp experiments. Cryo-electron microscopy (cryo-EM) structures of dVGACs closely align with the design models. Cryo-EM structures and molecular dynamics simulations suggest that the arginine constrictions undergo voltage-induced conformational changes, serving as both a voltage sensor and a selectivity filter as designed. Notably, the anion selectivity and voltage sensitivity of dVGACs can be tuned through targeted mutations for suppressing neuronal firing in situ. The ability to create ion channels with custom-designed conformational changes refreshes our insights into membrane biophysics and unveils diverse potential applications.
External linksCell / PubMed:41106381
MethodsEM (single particle)
Resolution2.9 - 3.72 Å
Structure data

39496

8yq5

EMDB-39496, PDB-8yq5:
Cryo-EM structure of a de novo designed transmembrane protein tmZC8-BTB
Method: EM (single particle) / Resolution: 3.3 Å

39497

8yq6

EMDB-39497, PDB-8yq6:
Cryo-EM structure of a de novo designed voltage-gated anion channel (dVGAC)
Method: EM (single particle) / Resolution: 2.9 Å

63538

9m05

EMDB-63538, PDB-9m05:
Cryo-EM structure of dVGAC complexed with iodide
Method: EM (single particle) / Resolution: 3.72 Å

Chemicals

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-IOD:
IODIDE ION

Source
  • synthetic construct (others)
KeywordsDE NOVO PROTEIN / De novo design / Ion channels

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