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Structure paper

TitleStructural basis for DNA replication by the African swine fever virus polymerase.
Journal, issue, pagesiScience, Vol. 28, Issue 12, Page 114155, Year 2025
Publish dateDec 19, 2025
AuthorsZiwei Hu / Yongjie SunKang / Zhiheng Liu / Lihong Huang / Wenbao Qi / Renhong Yan /
PubMed AbstractAfrican swine fever virus (ASFV) devastates swine herds and lacks widely available antivirals. We show that the ASFV DNA polymerase beta (Pol β; gene ) localizes to viral replication factories and ...African swine fever virus (ASFV) devastates swine herds and lacks widely available antivirals. We show that the ASFV DNA polymerase beta (Pol β; gene ) localizes to viral replication factories and is required for viral replication. Cryo-electron microscopy structures of Pol β in apo and double-stranded DNA (dsDNA)-bound states reveal pronounced conformational flexibility that enables transitions between functional states. Binding to dsDNA promotes higher-order oligomerization that is essential for catalytic activity, as demonstrated by structure-guided mutagenesis and biochemical assays. These findings define the structural basis of ASFV genome synthesis, highlight the functional significance of Pol β during viral replication, and provide a framework for polymerase-targeted antiviral discovery.
External linksiScience / PubMed:41438038 / PubMed Central
MethodsEM (single particle)
Resolution2.53 - 2.93 Å
Structure data

62890

9l8t

EMDB-62890, PDB-9l8t:
The apo structure (State 1) of African Swine Fever Virus DNA polymerase
Method: EM (single particle) / Resolution: 2.92 Å

62891

9l8u

EMDB-62891, PDB-9l8u:
Cryo-EM structure of ASFV DNA polymerase in a DNA replication state
Method: EM (single particle) / Resolution: 2.53 Å

EMDB-65680: Cryo-EM map of ASFV DNA polymerase in apo state 2
Method: EM (single particle) / Resolution: 2.93 Å

Chemicals

ChemComp-DCP:
2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE

ChemComp-MG:
Unknown entry

Source
  • asfarviridae (virus)
  • african swine fever virus
KeywordsDNA BINDING PROTEIN / ASFV / DNA polymerase / Virus / DNA BINDING PROTEIN/DNA / DNA BINDING PROTEIN-DNA complex

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