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TitlePorcine serum maltase-glucoamylase: structure, kinetics, and inhibition.
Journal, issue, pagesJ Enzyme Inhib Med Chem, Vol. 41, Issue 1, Page 2612391, Year 2026
Publish dateJan 14, 2026
AuthorsKen Watanabe / Takayoshi Tagami / Chihiro Biwa / Masato Kawasaki / Naruhiko Adachi / Toshio Moriya / Toshiya Senda / Masayuki Okuyama /
PubMed AbstractMaltase-glucoamylase (MGAM) is a small-intestinal enzyme comprising two tandem α-glucosidase units, NtMGAM and CtMGAM, each capable of hydrolysing maltodextrins into glucose. MGAM serves as a ...Maltase-glucoamylase (MGAM) is a small-intestinal enzyme comprising two tandem α-glucosidase units, NtMGAM and CtMGAM, each capable of hydrolysing maltodextrins into glucose. MGAM serves as a therapeutic target for managing postprandial hyperglycaemia; comprehensive insights into its full-length three-dimensional structure and inhibitor kinetics remains limited. Here, we demonstrate that the α-glucosidase in porcine serum is comparable to that encoded by the MGAM gene. Using cryo-electron microscopy, we determined the complex structure of serum MGAM with the inhibitor acarviosyl-maltotriose (AC5), which was found to bind exclusively to the active sites of each unit, confirming the presence of independent catalytic sites. AC5 was shown to exhibit mixed-type inhibition towards full-length serum MGAM and competitive inhibition against both recombinant NtMGAM and CtMGAM. The apparent mixed-type inhibition can be more accurately attributed to dual competitive inhibition mechanisms. These findings contribute to the advancement of functional foods and therapeutic interventions for postprandial hyperglycaemia and type 2 diabetes.
External linksJ Enzyme Inhib Med Chem / PubMed:41534875 / PubMed Central
MethodsEM (single particle)
Resolution2.77 - 3.17 Å
Structure data

62652

9kz6

EMDB-62652, PDB-9kz6:
The cryo-EM structure of porcine serum MGAM
Method: EM (single particle) / Resolution: 3.17 Å

62653

9kz7

EMDB-62653, PDB-9kz7:
The cryo-EM structure of porcine serum MGAM bound with Acarviosyl-maltotriose.
Method: EM (single particle) / Resolution: 2.77 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • sus scrofa domesticus (domestic pig)
  • Sus scrofa (pig)
KeywordsHYDROLASE / alpha-glucosidase

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