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-Structure paper
| タイトル | Cryo-EM structure and dynamic basis of phosphate uptake by PHT1 in rice. |
|---|---|
| ジャーナル・号・ページ | Dev Cell, Vol. 61, Issue 1, Page 164-177.e6, Year 2026 |
| 掲載日 | 2026年1月14日 |
著者 | Zhangmeng Du / Zeyuan Guan / Hai Liu / Jie Zhang / Haitao He / Zhiwen Zheng / Wenhui Zhang / Lihuan Jiang / Jiaqi Zuo / Yan Liu / Beijing Wan / Haifu Tu / Faming Dong / Xuelei Lai / Lizhong Xiong / Ping Yin / Shaowu Xue / Yanke Chen / Zhu Liu / ![]() |
| PubMed 要旨 | Phosphorus is an essential macronutrient for plants, primarily absorbed from the soil as inorganic phosphate (Pi) through root-located Pi transporters. Despite decades of research into these ...Phosphorus is an essential macronutrient for plants, primarily absorbed from the soil as inorganic phosphate (Pi) through root-located Pi transporters. Despite decades of research into these transporters as targets for developing Pi-efficient crops, their mechanisms for Pi import remain poorly understood. Here, we present the cryo-electron microscopy (cryo-EM) structures of the rice Pi importer OsPHT1;11 in both Pi-bound and unbound forms, characterize its conformational dynamics, and demonstrate how these dynamics contribute to its transport function. Pi is recognized through conserved residues found in plants, with its translocation facilitated by a typical alternating-access mechanism. Single-molecule fluorescence resonance energy transfer (smFRET) analyses show that this transporter undergoes dynamic conformational fluctuations, which are differentially linked to its Pi transport capability, with a predominance of extracellular open conformations favoring Pi transport, while more populated intracellular open conformations hinder it. These findings highlight key conformational determinants of transport activity and provide mechanistic insights into Pi uptake in plants. |
リンク | Dev Cell / PubMed:41005295 |
| 手法 | EM (単粒子) |
| 解像度 | 3.3 - 3.4 Å |
| 構造データ | EMDB-62441, PDB-9kmq: EMDB-62480, PDB-9kou: |
| 化合物 | ![]() ChemComp-PO4: |
| 由来 |
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キーワード | TRANSPORT PROTEIN / phosphorus / rice / uptake |
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