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TitleCrystal and Cryo-EM structure of PPL, a novel hexameric R-type lectin from the poisonous mushroom Pleurocybella porrigens.
Journal, issue, pagesGlycobiology, Vol. 36, Issue 1, Year 2025
Publish dateDec 17, 2025
AuthorsDaisuke Adachi / Naito Ishimoto / Kenji Mizutani / Katsuya Takahashi / Reiji Kubota / Haruka Kawabata / Sam-Yong Park / Laurens Vandebroek / Arnout R D Voet / Masao Yamada / Yasuhiro Ozeki / Yuki Fujii / Hideaki Fujita / Jeremy R H Tame / Kenichi Kamata /
PubMed AbstractPleurocybella porrigens is a mushroom that grows widely around the temperate northern hemisphere, and was once considered edible, especially in Japan. Following a number of deaths in 2004, ...Pleurocybella porrigens is a mushroom that grows widely around the temperate northern hemisphere, and was once considered edible, especially in Japan. Following a number of deaths in 2004, investigations revealed the presence of various toxins, including a lectin (PPL) that apparently survives cooking and enters the bloodstream via the stomach. We have cloned PPL and solved its structure by X-ray crystallography and cryo-EM. We report the sugar binding properties of this β-trefoil lectin, which has a novel hexameric structure.
External linksGlycobiology / PubMed:41342409
MethodsEM (single particle) / X-ray diffraction
Resolution2 - 3.24 Å
Structure data

62399

9kl3

EMDB-62399, PDB-9kl3:
CryoEM structure of Pleurocybella porrigens lectin (PPL) in complex with GalNAc
Method: EM (single particle) / Resolution: 3.24 Å

PDB-9kl2:
Crystal structure of Pleurocybella porrigens lectin (PPL) in complex with GalNAc
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-A2G:
2-acetamido-2-deoxy-alpha-D-galactopyranose

ChemComp-NO3:
NITRATE ION

ChemComp-HOH:
WATER

Source
  • pleurocybella porrigens (fungus)
KeywordsSUGAR BINDING PROTEIN / trefoil / lectin

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