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Structure paper

Title5' extended gRNA increases the cleavage activity of SuperFi-Cas9
Journal, issue, pagesTo Be Published
Publish dateOct 21, 2024 (structure data deposition date)
AuthorsZheng R / Ma LJ
External linksSearch PubMed
MethodsEM (single particle)
Resolution3.27 - 4.4 Å
Structure data

62054

9k4c

EMDB-62054, PDB-9k4c:
SuperFi Cas9 - 22nt sgRNA - DNA ternary complex
Method: EM (single particle) / Resolution: 3.84 Å

62055

9k4d

EMDB-62055, PDB-9k4d:
SuperFi Cas9 - 20nt sgRNA - DNA ternary complex
Method: EM (single particle) / Resolution: 3.53 Å

62056

9k4e

EMDB-62056, PDB-9k4e:
SuperFi Cas9 - mismatch 22nt sgRNA - DNA ternary complex class1
Method: EM (single particle) / Resolution: 3.3 Å

62057

9k4f

EMDB-62057, PDB-9k4f:
SuperFi Cas9 - mismatch 22nt sgRNA - DNA ternary complex class2
Method: EM (single particle) / Resolution: 3.27 Å

62059

9k4h

EMDB-62059, PDB-9k4h:
SuperFi Cas9 - mismatch 22nt sgRNA - DNA ternary complex class3
Method: EM (single particle) / Resolution: 3.36 Å

65730

9w7q

EMDB-65730, PDB-9w7q:
SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class A
Method: EM (single particle) / Resolution: 3.79 Å

65732

9w7t

EMDB-65732, PDB-9w7t:
SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class B
Method: EM (single particle) / Resolution: 3.91 Å

65733

9w7u

EMDB-65733, PDB-9w7u:
SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class C
Method: EM (single particle) / Resolution: 3.83 Å

65734

9w7v

EMDB-65734, PDB-9w7v:
SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class D
Method: EM (single particle) / Resolution: 3.81 Å

65771

9w9d

EMDB-65771, PDB-9w9d:
SuperFi Cas9 - 22nt sgRNA - DNA ternary complex
Method: EM (single particle) / Resolution: 4.4 Å

65809

9wa9

EMDB-65809, PDB-9wa9:
SuperFi Cas9 - 22nt sgRNA - DNA ternary complex Class B
Method: EM (single particle) / Resolution: 4.12 Å

65810

9waa

EMDB-65810, PDB-9waa:
SuperFi Cas9 - 22nt sgRNA - DNA ternary complex Class C
Method: EM (single particle) / Resolution: 4.17 Å

65827

9waw

EMDB-65827, PDB-9waw:
SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class E
Method: EM (single particle) / Resolution: 3.95 Å

Source
  • streptococcus pyogenes (bacteria)
  • synthetic construct (others)
  • streptococcus pyogenes serotype m1 (bacteria)
KeywordsIMMUNE SYSTEM/DNA/RNA / Complex / cas / SuperFi cas9 / IMMUNE SYSTEM / IMMUNE SYSTEM-DNA-RNA complex

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