EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	KIDINS220 and InsP8 safeguard the stepwise regulation of phosphate exporter XPR1.
ジャーナル・号・ページ	Mol Cell, Vol. 85, Issue 17, Page 3209-33224.e8, Year 2025
掲載日	2025年9月4日
著者	Xiaojie Wang / Zhongjian Bai / Ciara Wallis / Huanchen Wang / Yaoyao Han / Ruitao Jin / Mingguang Lei / Tian Yang / Chunfang Gu / Henning Jessen / Stephen Shears / Yadong Sun / Ben Corry / Yixiao Zhang /
PubMed 要旨	XPR1 is emerging as the only known inorganic phosphate (Pi) exporter in humans, critical for Pi homeostasis, with its activity stimulated by inositol pyrophosphate InsP8 and regulated by neuronal ...XPR1 is emerging as the only known inorganic phosphate (Pi) exporter in humans, critical for Pi homeostasis, with its activity stimulated by inositol pyrophosphate InsP8 and regulated by neuronal scaffold protein KIDINS220. Our structural studies reveal that InsP8 specifically activates XPR1 in a stepwise manner, involving profound SYG1/PHO/XPR1 (SPX) domain movements. Each XPR1 subunit functions with four gating states, in which Pi permeates a constriction site via a "knock-kiss-kick" process. By contrast, KIDINS220 delicately stabilizes XPR1 in a closed conformation through multiple mechanisms, one of which involves trapping the XPR1 α1 helix-critical for InsP8 binding-within an interaction hub. InsP8 serves as a key to release KIDINS220's restraint, reinforcing a "key-to-locks" mechanism to safeguard the stepwise activation. Additionally, our study provides direct structural insights into XPR1-associated neuronal disorders and highlights the evolutionary conservation and divergence among XPR1 orthologs, offering a comprehensive understanding of Pi homeostasis across species.
リンク	Mol Cell / PubMed:40858110 / PubMed Central
手法	EM (単粒子)
解像度	3.12 - 7.52 Å
構造データ	61859 9jxd EMDB-61859, PDB-9jxd: Cryo-EM structure of human XPR1 in apo state 手法: EM (単粒子) / 解像度: 3.29 Å 61860 9jxe EMDB-61860, PDB-9jxe: Cryo-EM structure of apo human XPR1, class 1, with one visible SPX domain 手法: EM (単粒子) / 解像度: 3.85 Å 61861 9jxf EMDB-61861, PDB-9jxf: Cryo-EM structure of apo human XPR1, class 2, with asymmetrically dimerized SPX domains 手法: EM (単粒子) / 解像度: 4.31 Å 61862 9jxg EMDB-61862, PDB-9jxg: Cryo-EM structure of apo human XPR1, class 3, with symmetrically dimerized SPX domains 手法: EM (単粒子) / 解像度: 3.75 Å 61863 9jxh EMDB-61863, PDB-9jxh: Cryo-EM structure of human XPR1 in complex with InsP8 手法: EM (単粒子) / 解像度: 3.15 Å 61864 9jxi EMDB-61864, PDB-9jxi: Cryo-EM structure of human XPR1 with InsP8 in an 'arch-like' intermediate state of the SPX domain 手法: EM (単粒子) / 解像度: 3.31 Å 61865 9jxj EMDB-61865, PDB-9jxj: Cryo-EM structure of human XPR1 in a closed state at both the extracellular and intracellular gates, obtained through local refinement 手法: EM (単粒子) / 解像度: 3.22 Å 61866 9jxk EMDB-61866, PDB-9jxk: Cryo-EM structure of human XPR1 in a closed state at the intracellular gate and an open state at the extracellular gate, obtained through local refinement 手法: EM (単粒子) / 解像度: 3.38 Å 61867 9jxl EMDB-61867, PDB-9jxl: Cryo-EM structure of human XPR1 in an open state at the intracellular gate and a closed state at the extracellular gate, obtained through local refinement 手法: EM (単粒子) / 解像度: 3.23 Å 61868 9jxm EMDB-61868, PDB-9jxm: Cryo-EM structure of one subunit of the human XPR1 homodimer with both the intracellular and extracellular gates in an open state, obtained through local refinement 手法: EM (単粒子) / 解像度: 3.48 Å 61869 9jxn EMDB-61869, PDB-9jxn: Cryo-EM structure of human XPR1-R459C 手法: EM (単粒子) / 解像度: 3.12 Å 61870 9jxo EMDB-61870, PDB-9jxo: Cryo-EM structure of human XPR1-G621A 手法: EM (単粒子) / 解像度: 3.48 Å 61871 9jxp EMDB-61871, PDB-9jxp: Cryo-EM structure of human XPR1-R570L 手法: EM (単粒子) / 解像度: 3.53 Å EMDB-61872: Cryo-EM structure of XPR1-KIDINS220 complex 手法: EM (単粒子) / 解像度: 3.44 Å EMDB-61873: Local refinement of SPX and ARs in XPR1-KIDINS220 complex 手法: EM (単粒子) / 解像度: 3.54 Å EMDB-61874: Local refinement of additional KIDINS220 TM-helix in XPR1-KIDINS220 complex 手法: EM (単粒子) / 解像度: 3.75 Å 61875 9jxq EMDB-61875, PDB-9jxq: Complex of XPR1-KIDINS220 手法: EM (単粒子) / 解像度: 3.44 Å EMDB-61876: Cryo-EM structure of XPR1-KIDINS220-InsP8 complex 手法: EM (単粒子) / 解像度: 4.47 Å 61877 9jxr EMDB-61877, PDB-9jxr: Cryo-EM structure of Drosophila melanogaster PXo-G604A 手法: EM (単粒子) / 解像度: 3.53 Å EMDB-61878: Cryo-EM structure of Drosophila melanogaster PXo 手法: EM (単粒子) / 解像度: 5.85 Å EMDB-61879: Cryo-EM structure of PHO1 手法: EM (単粒子) / 解像度: 7.52 Å
化合物	ChemComp-I8P: (1R,3S,4R,5S,6R)-2,4,5,6-tetrakis(phosphonooxy)cyclohexane-1,3-diyl bis[trihydrogen (diphosphate)] ChemComp-HOH: WATER ChemComp-PO4: PHOSPHATE ION
由来	homo sapiens (ヒト) drosophila melanogaster (キイロショウジョウバエ)
キーワード	TRANSPORT PROTEIN / phosphate channel;phosphate exporter;PFBC / phosphate channel