EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures and mechanisms of the ABC transporter ABCB1 from Arabidopsis thaliana.
ジャーナル・号・ページ	Structure, Vol. 33, Issue 5, Page 903-915.e5, Year 2025
掲載日	2025年5月1日
著者	Qian Chen / Li Zhu / Sufen Zhang / Shuai Qiao / Zhong Jie Ding / Shao Jian Zheng / Jiangtao Guo / Nannan Su /
PubMed 要旨	The Arabidopsis thaliana auxin transporter ABCB1 plays a fundamental role in the regulation of plant growth and development. While its homolog ABCB19 was previously shown to transport ...The Arabidopsis thaliana auxin transporter ABCB1 plays a fundamental role in the regulation of plant growth and development. While its homolog ABCB19 was previously shown to transport brassinosteroids (BR), another class of essential hormones, the ability of ABCB1 to mediate BR transport has remained unexplored. In this study we show that ABCB1 also transports brassinosteroids with an in vitro brassinolide (BL) transport assay. Using single-particle cryo-electron microscopy, we determined ABCB1 structures in multiple inward-facing conformations in the apo state, ANP-bound state, BL-bound state, and the both BL- and ANP-bound state. BL binds to the large cavity of two transmembrane domains, inducing a slight conformational change. Additionally, we obtained the structure of ABCB1 in an outward-facing conformation. By comparing these different conformations, we elucidated the possible mechanism of hormone transport by ABCB1. These high-resolution structures help us to understand the structural basis for hormone recognition and transport mechanisms of ABCB1.
リンク	Structure / PubMed:40101709
手法	EM (単粒子)
解像度	3.1 - 3.4 Å
構造データ	61827 9juj EMDB-61827, PDB-9juj: Structure of Arabidopsis thaliana ABCB1 in the inward-facing conformation 手法: EM (単粒子) / 解像度: 3.4 Å 61828 9juk EMDB-61828, PDB-9juk: Structure of Arabidopsis thaliana ABCB1 with AMP-PNP bound in the inward-facing conformation 手法: EM (単粒子) / 解像度: 3.3 Å 61829 9jul EMDB-61829, PDB-9jul: Structure of Arabidopsis thaliana ABCB1 with brassinolide bound in the inward-facing conformation 手法: EM (単粒子) / 解像度: 3.1 Å 61830 9jum EMDB-61830, PDB-9jum: Structure of Arabidopsis thaliana ABCB1 with brassinolide and AMP-PNP bound in the inward-facing conformation 手法: EM (単粒子) / 解像度: 3.1 Å 61831 9jun EMDB-61831, PDB-9jun: Structure of Arabidopsis thaliana ABCB1 in the inward-facing conformation under IAA condition 手法: EM (単粒子) / 解像度: 3.4 Å 61832 9juo EMDB-61832, PDB-9juo: Structure of Arabidopsis thaliana ABCB1 with AMP-PNP bound in the inward-facing conformation under IAA condition 手法: EM (単粒子) / 解像度: 3.1 Å 61833 9jup EMDB-61833, PDB-9jup: Structure of Arabidopsis thaliana ABCB1 with AMP-PNP bound in the outward-facing conformation 手法: EM (単粒子) / 解像度: 3.1 Å
化合物	ChemComp-MG: Unknown entry ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, エネルギー貯蔵分子類似体YM ChemComp-BLD*: Brassinolide
由来	arabidopsis thaliana (シロイヌナズナ)
キーワード	TRANSPORT PROTEIN / ABCB1 / auxin transport / brassinolide (BL) transport