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-Structure paper
| タイトル | Structural polymorphism of two-dimensional lattices assembled from baculoviral capsid proteins. |
|---|---|
| ジャーナル・号・ページ | Virol Sin, Vol. 40, Issue 6, Page 935-945, Year 2025 |
| 掲載日 | 2025年11月19日 |
著者 | Kexing Tian / Heya Na / Yan Fu / Tingting Chong / Chao Leng / Fanxing Meng / Yaozhou Liang / Manli Wang / Zhihong Hu / Xi Wang / Guibo Rao / Sheng Cao / ![]() |
| PubMed 要旨 | Protein nanotubes (PNTs) can be regarded as two-dimensional (2D) lattices with p1 or p2 symmetry rolled into tubes. However, attempts to re-assemble their building blocks into stable 2D nanomaterials ...Protein nanotubes (PNTs) can be regarded as two-dimensional (2D) lattices with p1 or p2 symmetry rolled into tubes. However, attempts to re-assemble their building blocks into stable 2D nanomaterials often fail. Here, starting from two baculoviral capsid proteins, we screened protein variants for the in vitro assembly of various nanotubes and nanosheets. These high-order assemblies were structurally characterized by cryo-electron microscopy techniques. Interfacial analysis of three groups of PNTs revealed that helical heterogeneity is largely the result of the redundancy of p2 symmetry-related contacting interfaces. The assembled nanosheets showed similar interfacial networks to their nanotubular counterparts. In addition, foreign macromolecules could be efficiently displayed on the size-controllable double-layered nanosheets. This study sheds light on the rational design of flexible nanosheets, and it also provides novel 2D protein scaffolds for developing biocompatible materials. |
リンク | Virol Sin / PubMed:41265796 |
| 手法 | EM (らせん対称) / EM (単粒子) |
| 解像度 | 2.8 - 6.6 Å |
| 構造データ | ![]() EMDB-39489: Cryo-EM structure of H11 nanotubes assembled from baculovirus capsid protein ![]() EMDB-39492: Cryo-EM structure of H12 nanotubes assembled from baculovirus capsid protein ![]() EMDB-61711: H14 nanotubes assembled from baculovirus capsid protein EMDB-61712, PDB-9jpr: EMDB-61713, PDB-9jps: EMDB-61714, PDB-9jpt: ![]() EMDB-61996: A7 nanotubes assembled from baculovirus capsid protein EMDB-61997, PDB-9k2o: |
| 由来 |
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キーワード | VIRAL PROTEIN / capsid protein / self-assembly / Baculovirus / nanotube |
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helicoverpa armigera nucleopolyhedrovirus (ウイルス)
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