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TitleCorrelative voltage imaging and cryo-electron tomography bridge neuronal activity and molecular structure.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 9378, Year 2025
Publish dateOct 23, 2025
AuthorsMingyu Jung / Gwanho Ko / Dongsung Lim / Seonghoon Kim / Sojeong Kim / Young-Joon Kim / Myunghwan Choi / Soung-Hun Roh /
PubMed AbstractNeurons exhibit varying electrophysiological properties due to dynamic changes in spatiotemporal molecular networks. In situ cryo-electron tomography (cryo-ET) provides advantages for high-resolution ...Neurons exhibit varying electrophysiological properties due to dynamic changes in spatiotemporal molecular networks. In situ cryo-electron tomography (cryo-ET) provides advantages for high-resolution visualization of macromolecular complexes within their cellular context. Although correlation with fluorescent labeling allows cryo-ET to target specific cellular regions, it does not adequately reflect the electrophysiological properties of heterogeneous neurons. To bridge high-resolution molecular imaging with electrophysiological properties of individual neurons, we develop a Correlative Voltage Imaging and cryo-ET (CoVET) technique. The nondestructive nature of voltage imaging is compatible with cryo-ET, enabling a direct correlation between neuronal electrophysiology and molecular structures. Neurons are clustered based on their electrophysiological properties, allowing for single-cell-guided structural analysis using cryo-ET. We analyze the translational landscapes of individual neurons and find distinct structural characteristics and spatial networks among ribosomes from different electrophysiological clusters. Our results highlight the importance of the correlation between the electrophysiological properties and molecular structures.
External linksNat Commun / PubMed:41130945 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution7.8 - 12.7 Å
Structure data

EMDB-61318: Ribosome from rattus norvegicus hippocampal primary neuron
Method: EM (subtomogram averaging) / Resolution: 7.8 Å

EMDB-61633: Decoding1 states from rattus norvegicus 80S ribosome
Method: EM (subtomogram averaging) / Resolution: 7.9 Å

EMDB-61634: Decoding2 states from rattus norvegicus 80S ribosome
Method: EM (subtomogram averaging) / Resolution: 8.2 Å

EMDB-61635: DecodingZ states from rattus norvegicus 80S ribosome
Method: EM (subtomogram averaging) / Resolution: 11.0 Å

EMDB-61636: Hibernating states from rattus norvegicus 80S ribosome
Method: EM (subtomogram averaging) / Resolution: 8.8 Å

EMDB-61637: Pre states with factor from rattus norvegicus 80S ribosome
Method: EM (subtomogram averaging) / Resolution: 12.0 Å

EMDB-61638: Pre states without factor from rattus norvegicus 80S ribosome
Method: EM (subtomogram averaging) / Resolution: 12.7 Å

EMDB-61639: Rotated1 states without factor from rattus norvegicus 80S ribosome
Method: EM (subtomogram averaging) / Resolution: 8.6 Å

EMDB-61640: Rotated2 states without factor from rattus norvegicus 80S ribosome
Method: EM (subtomogram averaging) / Resolution: 8.1 Å

Source
  • Rattus norvegicus (Norway rat)

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