EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Antimalarial drug artemisinin stabilizes PfRACK1 binding to the ribosome.
ジャーナル・号・ページ	Structure, Vol. 33, Issue 8, Page 1386-11397.e5, Year 2025
掲載日	2025年8月7日
著者	Ka Diam Go / Xin-Fu Yan / Grennady Wirjanata / Rya Ero / Samuel Pazicky / Jerzy Dziekan / Seth Tjia / Julien Lescar / Zbynek Bozdech / Yong-Gui Gao /
PubMed 要旨	Artemisinin and its derivatives represent the core agents in artemisinin combination therapies that are the current frontline treatment for P. falciparum and P. vivax malaria infections. Artemisinins ...Artemisinin and its derivatives represent the core agents in artemisinin combination therapies that are the current frontline treatment for P. falciparum and P. vivax malaria infections. Artemisinins are known to bind a wide array of proteins that disrupt the parasite's cellular physiology. Here, we show that artemisinins' cytotoxic activity involves structural alteration of key P. falciparum macromolecular complexes, including the ribosome, proteasome, and T-complex. The structural analysis revealed that, following artemisinin treatment, a larger population of Pf80S ribosomes binds PfRACK1. Unlike in most eukaryotes, PfRACK1 does not interact with the C-terminal tail of the r-protein uS3 that in Plasmodium is truncated. This likely suggests an evolved role of uS3 in facilitating RACK1-mediated translational regulation, which would potentially benefit the parasite under certain conditions. Stabilization of RACK1 ribosome interaction likely contributes to artemisinins' mode of action.
リンク	Structure / PubMed:40480224
手法	EM (単粒子)
解像度	2.36 - 3.98 Å
構造データ	EMDB-61258: High-resolution cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to E-tRNA (consensus map) 手法: EM (単粒子) / 解像度: 2.46 Å EMDB-61259: High-resolution cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to E-tRNA (Large Ribosomal Subunit) 手法: EM (単粒子) / 解像度: 2.4 Å EMDB-61260: High-resolution cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to E-tRNA (Small Ribosomal Subunit Body) 手法: EM (単粒子) / 解像度: 2.5 Å EMDB-61261: High-resolution cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to E-tRNA (Small Ribosomal Subunit Head) 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-61262: High-resolution cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to RACK1 and E-tRNA (consensus map) 手法: EM (単粒子) / 解像度: 2.45 Å EMDB-61263: High-resolution cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to RACK1 and E-tRNA (Large Ribosomal Subunit) 手法: EM (単粒子) / 解像度: 2.36 Å EMDB-61264: High-resolution cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to RACK1 and E-tRNA (Small Ribosomal Subunit Body) 手法: EM (単粒子) / 解像度: 2.47 Å EMDB-61265: High-resolution cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to RACK1 and E-tRNA (Small Ribosomal Subunit Head) 手法: EM (単粒子) / 解像度: 2.54 Å 61272 9j9h EMDB-61272, PDB-9j9h: High-resolution cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to RACK1 and E-tRNA 手法: EM (単粒子) / 解像度: 2.45 Å 61273 9j9i EMDB-61273, PDB-9j9i: High-resolution cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to E-tRNA 手法: EM (単粒子) / 解像度: 2.46 Å EMDB-61274: Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to RACK1 and E-tRNA 手法: EM (単粒子) / 解像度: 3.98 Å EMDB-61275: Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to E-tRNA 手法: EM (単粒子) / 解像度: 3.37 Å
化合物	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry
由来	plasmodium falciparum 3d7 (マラリア病原虫)
キーワード	RIBOSOME / cryo-EM / Plasmodium falciparum