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TitleMPicker: visualizing and picking membrane proteins for cryo-electron tomography.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 472, Year 2025
Publish dateJan 8, 2025
AuthorsXiaofeng Yan / Shudong Li / Weilin Huang / Hao Wang / Tianfang Zhao / Mingtao Huang / Niyun Zhou / Yuan Shen / Xueming Li /
PubMed AbstractAdvancements in cryo-electron tomography (cryoET) allow the structure of macromolecules to be determined in situ, which is crucial for studying membrane protein structures and their interactions in ...Advancements in cryo-electron tomography (cryoET) allow the structure of macromolecules to be determined in situ, which is crucial for studying membrane protein structures and their interactions in the cellular environment. However, membranes are often highly curved and have a strong contrast in cryoET tomograms, which masks the signals from membrane proteins. These factors pose difficulties in observing and revealing the structures of membrane proteins in situ. Here, we report a membrane-flattening method and the corresponding software, MPicker, designed for the visualization, localization, and orientation determination of membrane proteins in cryoET tomograms. This method improves the visualization of proteins on and around membranes by generating a flattened tomogram that eliminates membrane curvature and reduces the spatial complexity of membrane protein analysis. In MPicker, we integrated approaches for automated particle picking and coarse alignment of membrane proteins for sub-tomogram averaging. MPicker was tested on tomograms of various cells to evaluate the method for visualizing, picking, and analyzing membrane proteins.
External linksNat Commun / PubMed:39774981 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution24.2 Å
Structure data

EMDB-61019: Subtomogram averaging of the C2S2M2L2-type PSII-LHCII supercomplex from Chlamydomonas reihardtii
Method: EM (subtomogram averaging) / Resolution: 24.2 Å

Source
  • Chlamydomonas reihardtii (plant)

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