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-Structure paper
| タイトル | Metabolically regulated proteasome supramolecular organization in situ. |
|---|---|
| ジャーナル・号・ページ | Cell, Vol. 189, Issue 4, Page 1153-1169.e16, Year 2026 |
| 掲載日 | 2026年2月19日 |
 著者 | Xiaomeng Tang / Lu Qu / Florian Wilfling / Florian Beck / Oliver P Ernst / Brenda A Schulman / Wolfgang Baumeister / Cordula Enenkel /   |
| PubMed 要旨 | Many proteins localize in membraneless organelles. However, understanding the steps along membraneless organelle formation-and the structural impact on granule constituents-has been hindered by ...Many proteins localize in membraneless organelles. However, understanding the steps along membraneless organelle formation-and the structural impact on granule constituents-has been hindered by limited resolution of intracellular data. We address these challenges through in situ cryo-electron tomography (cryo-ET) along with formation of yeast proteasome storage granules (PSGs). During the transition from proliferation to quiescence, doubly capped 26S proteasomes arrested in an inactive state arrange into ∼7.5 MDa trimeric units, dispersed in the nucleoplasm and congregated along the nuclear envelope near the nuclear pore. 9-Å-resolution cryo-ET structures reveal that cytoplasmic PSGs formed in various energy-limiting conditions are paracrystalline arrays of bundled fibers, assembled from stacking of proteasome trimers. The paracrystalline arrangement maintains a pool of fully assembled inactive 26S proteasomes that are released in energy-rich conditions. Overall, our data reveal structural steps along the assembly of an intracellular membraneless organelle in situ and quinary structure formation controlling a major eukaryotic regulatory machine. |
 リンク | Cell / PubMed:41605212 |
| 手法 | EM (トモグラフィー) / EM (サブトモグラム平均) |
| 解像度 | 9.1 - 27.4 Å |
| 構造データ |  EMDB-60949: In situ cryo-electron tomogram of 4days WT cytoplasm 1  EMDB-60950: In situ cryo-electron tomogram of 4days WT nucleus (PSG at NE)  EMDB-60951: In situ cryo-electron tomogram of 4days WT nucleus (trimer close to NE)  EMDB-60952: In situ cryo-electron tomogram of 4days rpn13null nucleus  EMDB-60953: In situ cryo-electron tomogram of 1day WT nucleus  EMDB-60955: In situ cryo-electron tomogram of 4days rpn9deltaN nucleus  EMDB-60957: In situ cryo-electron tomogram of 4days WT cell EMDB-60960, PDB-9iwr:  EMDB-60961: 26S proteasome trimer close to nuclear envelope  EMDB-64627: In situ cryo-electron tomogram of 4days rpn9 surface mutant nucleus  EMDB-64628: In situ cryo-electron tomogram of 18h nucleus  EMDB-64629: In situ cryo-electron tomogram of 4days WT cytoplasm 3  EMDB-64630: In situ cryo-electron tomogram of 4days glucose 1h WT nucleus  EMDB-64631: In situ cryo-electron tomogram of 4days glucose control WT nucleus  EMDB-64632: In situ cryo-electron tomogram of SA 1day WT cytoplasm 1  EMDB-64633: In situ cryo-electron tomogram of SA 1day WT cytoplasm 2  EMDB-64634: In situ cryo-electron tomogram of 4days mlp1delta mlp2delta nucleus  EMDB-64635: In vitro cryo-electron tomogram of 4days WT purified  EMDB-64636: In situ cryo-electron tomogram of 4days rpn9deltaN nucleus |
| 由来 |
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 キーワード | HYDROLASE / proteasome / trimer / complex / PSG / paracrystalline |
saccharomyces cerevisiae s288c (パン酵母)