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TitleDeciphering the assembly process of PQQ dependent methanol dehydrogenase.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 6672, Year 2025
Publish dateJul 19, 2025
AuthorsHaichuan Zhou / Junqing Sun / Jian Cheng / Min Wu / Jie Bai / Qian Li / Jie Shen / Manman Han / Chen Yang / Liangpo Li / Yuwan Liu / Qichen Cao / Weidong Liu / Haixia Xiao / Hongjun Dong / Feng Gao / Huifeng Jiang /
PubMed AbstractPyrroloquinoline quinone (PQQ)-dependent methanol dehydrogenases (MDHs), the periplasmic metalloenzymes in Gram-negative methylotrophic bacteria, play a pivotal role in methane and methanol bio- ...Pyrroloquinoline quinone (PQQ)-dependent methanol dehydrogenases (MDHs), the periplasmic metalloenzymes in Gram-negative methylotrophic bacteria, play a pivotal role in methane and methanol bio-utilization. Although the structures of many PQQ-dependent MDHs have been resolved, including the canonical heterotetrameric enzymes composed of two MxaF and two MxaI subunits with a molecule of PQQ and a calcium ion in the active site in MxaF, the biogenesis of these enzymes remains elusive. Here, we characterize a chaperone, MxaJ, responsible for PQQ incorporation by reconstructing a PQQ-dependent MDH assembly system in Escherichia coli. Using cryo-electron microscopy, we capture the structures of the intermediate complexes formed by the chaperone MxaJ and catalytic subunit MxaF during PQQ-dependent MDH maturation, revealing a chaperone-mediated molecular mechanism of cofactor incorporation. These findings not only advance our understanding on the biogenesis of PQQ-dependent MDH, but also provide an alternative engineering way for methane and methanol bioconversion.
External linksNat Commun / PubMed:40683858 / PubMed Central
MethodsEM (single particle)
Resolution2.78 - 2.81 Å
Structure data

60838

9ism

EMDB-60838, PDB-9ism:
Cryo-EM structure of MxaF/MxaJ complex
Method: EM (single particle) / Resolution: 2.78 Å

60840

9iso

EMDB-60840, PDB-9iso:
Cryo-EM structure of MxaF/MxaJ/PQQ complex
Method: EM (single particle) / Resolution: 2.81 Å

Chemicals

ChemComp-PQQ:
PYRROLOQUINOLINE QUINONE

Source
  • methylorubrum extorquens (bacteria)
KeywordsPROTEIN BINDING / MxaF/MxaJ / MxaF/MxaJ/PQQ

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