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TitleArchitecture and functional regulation of a plant PSII-LHCII megacomplex.
Journal, issue, pagesSci Adv, Vol. 10, Issue 50, Page eadq9967, Year 2024
Publish dateDec 13, 2024
AuthorsJianyu Shan / Dariusz M Niedzwiedzki / Rupal S Tomar / Zhenfeng Liu / Haijun Liu /
PubMed AbstractPhotosystem II (PSII) splits water in oxygenic photosynthesis on Earth. The structure and function of the CSM-type PSII-LHCII (light-harvesting complex II) megacomplexes from the wild-type and PsbR- ...Photosystem II (PSII) splits water in oxygenic photosynthesis on Earth. The structure and function of the CSM-type PSII-LHCII (light-harvesting complex II) megacomplexes from the wild-type and PsbR-deletion mutant plants are studied through electron microscopy (EM), structural mass spectrometry, and ultrafast fluorescence spectroscopy [time-resolved fluorescence (TRF)]. The cryo-EM structure of a type I CSM megacomplex demonstrates that the three domains of PsbR bind to the stromal side of D1, D2, and CP43; associate with the single transmembrane helix of the redox active Cyt ; and stabilize the luminal extrinsic PsbP, respectively. This megacomplex, with PsbR and PsbY centered around the narrow interface between two dimeric PSII cores, provides the supramolecular structural basis that regulates the plastoquinone occupancy in Q site, excitation energy transfer, and oxygen evolution. PSII-LHCII megacomplexes (types I and II) and LHC aggregation levels in mutant were also interrogated and compared to wild-type plants through EM and picosecond TRF.
External linksSci Adv / PubMed:39671473 / PubMed Central
MethodsEM (single particle)
Resolution3.22 - 4.26 Å
Structure data

39860

8z9d

EMDB-39860, PDB-8z9d:
cryo-EM structure of PSII-LHCII megacomplex from spinach
Method: EM (single particle) / Resolution: 3.22 Å

EMDB-60801: The structure of C4S4M2 type PSII-LHCII megacomplex from Spinach
Method: EM (single particle) / Resolution: 3.71 Å

EMDB-60802: Focus refinement of PSII core in C4S4M2 type PSII-LHCII megacomplex from spinach
Method: EM (single particle) / Resolution: 3.22 Å

EMDB-60805: Focus refinement of M-24-29 subcomplex of C4S4M2 type PSII-LHCII megacomplex from Spinach
Method: EM (single particle) / Resolution: 4.26 Å

EMDB-60810: Focus refinement of CS type PSII-LHCII monomer in PSII-LHCII megacomplex from Spinach
Method: EM (single particle) / Resolution: 3.39 Å

Chemicals

ChemComp-OEX:
CA-MN4-O5 CLUSTER

ChemComp-FE2:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-CLA:
CHLOROPHYLL A

ChemComp-PHO:
PHEOPHYTIN A

ChemComp-BCR:
BETA-CAROTENE

ChemComp-PL9:
2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-CHL:
CHLOROPHYLL B

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

ChemComp-LMU:
DODECYL-ALPHA-D-MALTOSIDE / detergent*YM

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-BCT:
BICARBONATE ION

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

Source
  • spinacia oleracea (spinach)
KeywordsPHOTOSYNTHESIS / PSII / LHCII / megacomplex

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