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TitleStructures of PSI-FCPI from Thalassiosira pseudonana grown under high light provide evidence for convergent evolution and light-adaptive strategies in diatom FCPIs.
Journal, issue, pagesJ Integr Plant Biol, Vol. 67, Issue 4, Page 949-966, Year 2025
Publish dateDec 13, 2024
AuthorsYue Feng / Zhenhua Li / Yang Yang / Lili Shen / Xiaoyi Li / Xueyang Liu / Xiaofei Zhang / Jinyang Zhang / Fei Ren / Yuan Wang / Cheng Liu / Guangye Han / Xuchu Wang / Tingyun Kuang / Jian-Ren Shen / Wenda Wang /
PubMed AbstractDiatoms rely on fucoxanthin chlorophyll a/c-binding proteins (FCPs) for light harvesting and energy quenching under marine environments. Here we report two cryo-electron microscopic structures of ...Diatoms rely on fucoxanthin chlorophyll a/c-binding proteins (FCPs) for light harvesting and energy quenching under marine environments. Here we report two cryo-electron microscopic structures of photosystem I (PSI) with either 13 or five fucoxanthin chlorophyll a/c-binding protein Is (FCPIs) at 2.78 and 3.20 Å resolutions from Thalassiosira pseudonana grown under high light (HL) conditions. Among them, five FCPIs are stably associated with the PSI core, these include Lhcr3, RedCAP, Lhcq8, Lhcf10, and FCP3. The eight additional Lhcr-type FCPIs are loosely associated with the PSI core and detached under the present purification conditions. The pigments of this centric diatom showed a higher proportion of chlorophylls a, diadinoxanthins, and diatoxanthins; some of the chlorophyll as and diadinoxanthins occupy the locations of fucoxanthins found in the huge PSI-FCPI from another centric diatom Chaetoceros gracilis grown under low-light conditions. These additional chlorophyll as may form more energy transfer pathways and additional diadinoxanthins may form more energy dissipation sites relying on the diadinoxanthin-diatoxanthin cycle. These results reveal the assembly mechanism of FCPIs and corresponding light-adaptive strategies of T. pseudonana PSI-FCPI, as well as the convergent evolution of the diatom PSI-FCPI structures.
External linksJ Integr Plant Biol / PubMed:39670505
MethodsEM (single particle)
Resolution2.78 - 3.2 Å
Structure data

60032

8zeh

EMDB-60032, PDB-8zeh:
PSI-FCPI-L in Thalassiosira pseudonana
Method: EM (single particle) / Resolution: 2.78 Å

60044

8zet

EMDB-60044, PDB-8zet:
Tp-PSI-FCPI-S in Thalassiosira pseudonana
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-DD6:
(3S,3'R,5R,6S,7cis)-7',8'-didehydro-5,6-dihydro-5,6-epoxy-beta,beta-carotene-3,3'-diol

ChemComp-CLA:
CHLOROPHYLL A

ChemComp-KC1:
Chlorophyll c1

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-A86:
(3S,3'S,5R,5'R,6S,6'R,8'R)-3,5'-dihydroxy-8-oxo-6',7'-didehydro-5,5',6,6',7,8-hexahydro-5,6-epoxy-beta,beta-caroten-3'-

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-BCR:
BETA-CAROTENE

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

ChemComp-PQN:
PHYLLOQUINONE

ChemComp-ET4:
(1~{R})-3,5,5-trimethyl-4-[(1~{E},3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E})-3,7,12,16-tetramethyl-18-[(4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15-octaen-17-ynyl]cyclohex-3-en-1-ol

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

Source
  • thalassiosira pseudonana ccmp1335 (Diatom)
KeywordsPHOTOSYNTHESIS / photosystem I / Tp-PSI-FCPI-S in Thalassiosira pseudonana

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