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TitleCasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 111, Issue 18, Page 6618-6623, Year 2014
Publish dateMay 6, 2014
AuthorsMegan L Hochstrasser / David W Taylor / Prashant Bhat / Chantal K Guegler / Samuel H Sternberg / Eva Nogales / Jennifer A Doudna /
PubMed AbstractIn bacteria, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) DNA-targeting complex Cascade (CRISPR-associated complex for antiviral defense) uses CRISPR RNA ...In bacteria, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) DNA-targeting complex Cascade (CRISPR-associated complex for antiviral defense) uses CRISPR RNA (crRNA) guides to bind complementary DNA targets at sites adjacent to a trinucleotide signature sequence called the protospacer adjacent motif (PAM). The Cascade complex then recruits Cas3, a nuclease-helicase that catalyzes unwinding and cleavage of foreign double-stranded DNA (dsDNA) bearing a sequence matching that of the crRNA. Cascade comprises the CasA-E proteins and one crRNA, forming a structure that binds and unwinds dsDNA to form an R loop in which the target strand of the DNA base pairs with the 32-nt RNA guide sequence. Single-particle electron microscopy reconstructions of dsDNA-bound Cascade with and without Cas3 reveal that Cascade positions the PAM-proximal end of the DNA duplex at the CasA subunit and near the site of Cas3 association. The finding that the DNA target and Cas3 colocalize with CasA implicates this subunit in a key target-validation step during DNA interference. We show biochemically that base pairing of the PAM region is unnecessary for target binding but critical for Cas3-mediated degradation. In addition, the L1 loop of CasA, previously implicated in PAM recognition, is essential for Cas3 activation following target binding by Cascade. Together, these data show that the CasA subunit of Cascade functions as an essential partner of Cas3 by recognizing DNA target sites and positioning Cas3 adjacent to the PAM to ensure cleavage.
External linksProc Natl Acad Sci U S A / PubMed:24748111 / PubMed Central
MethodsEM (single particle)
Resolution9.0 - 20.0 Å
Structure data

EMDB-5929:
CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference
Method: EM (single particle) / Resolution: 9.0 Å

EMDB-5930:
CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference
Method: EM (single particle) / Resolution: 20.0 Å

Source
  • Escherichia coli K-12 (bacteria)
  • Enterobacteria phage P7 (virus)

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