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TitleCoordinated rearrangements between cytoplasmic and periplasmic domains of the membrane protein complex ExbB-ExbD of Escherichia coli.
Journal, issue, pagesStructure, Vol. 22, Issue 5, Page 791-797, Year 2014
Publish dateMay 6, 2014
AuthorsAleksandr Sverzhinsky / Lucien Fabre / Andrew L Cottreau / Damien M P Biot-Pelletier / Sofia Khalil / Mihnea Bostina / Isabelle Rouiller / James W Coulton /
PubMed AbstractGram-negative bacteria rely on the ExbB-ExbD-TonB system for the import of essential nutrients. Despite decades of research, the stoichiometry, subunit organization, and mechanism of action of the ...Gram-negative bacteria rely on the ExbB-ExbD-TonB system for the import of essential nutrients. Despite decades of research, the stoichiometry, subunit organization, and mechanism of action of the membrane proteins of the Ton system remain unclear. We copurified ExbB with ExbD as an ∼240 kDa protein-detergent complex, measured by light scattering and by native gels. Quantitative Coomassie staining revealed a stoichiometry of ExbB4-ExbD2. Negative stain electron microscopy and 2D analysis showed particles of ∼10 nm diameter in multiple structural states. Nanogold labeling identified the position of the ExbD periplasmic domain. Random conical tilt was used to reconstruct the particles in three structural states followed by sorting of the single particles and refinement of each state. The different states are interpreted by coordinated structural rearrangements between the cytoplasmic domain and the periplasmic domain, concordant with in vivo predictions.
External linksStructure / PubMed:24657092
MethodsEM (single particle)
Resolution21.0 - 27.0 Å
Structure data

EMDB-5901:
3D Reconstruction of Membrane Protein Complex ExbB4-ExbD2
Method: EM (single particle) / Resolution: 21.0 Å

EMDB-5902:
3D Reconstruction of Membrane Protein Complex ExbB4-ExbD2
Method: EM (single particle) / Resolution: 27.0 Å

EMDB-5903:
3D Reconstruction of Membrane Protein Complex ExbB4-ExbD2
Method: EM (single particle) / Resolution: 24.0 Å

Source
  • Escherichia coli (E. coli)

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