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-Structure paper
Title | Single-particle EM reveals the higher-order domain architecture of soluble guanylate cyclase. |
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Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 111, Issue 8, Page 2960-2965, Year 2014 |
Publish date | Feb 25, 2014 |
![]() | Melody G Campbell / Eric S Underbakke / Clinton S Potter / Bridget Carragher / Michael A Marletta / ![]() |
PubMed Abstract | Soluble guanylate cyclase (sGC) is the primary nitric oxide (NO) receptor in mammals and a central component of the NO-signaling pathway. The NO-signaling pathways mediate diverse physiological ...Soluble guanylate cyclase (sGC) is the primary nitric oxide (NO) receptor in mammals and a central component of the NO-signaling pathway. The NO-signaling pathways mediate diverse physiological processes, including vasodilation, neurotransmission, and myocardial functions. sGC is a heterodimer assembled from two homologous subunits, each comprised of four domains. Although crystal structures of isolated domains have been reported, no structure is available for full-length sGC. We used single-particle electron microscopy to obtain the structure of the complete sGC heterodimer and determine its higher-order domain architecture. Overall, the protein is formed of two rigid modules: the catalytic dimer and the clustered Per/Art/Sim and heme-NO/O2-binding domains, connected by a parallel coiled coil at two hinge points. The quaternary assembly demonstrates a very high degree of flexibility. We captured hundreds of individual conformational snapshots of free sGC, NO-bound sGC, and guanosine-5'-[(α,β)-methylene]triphosphate-bound sGC. The molecular architecture and pronounced flexibility observed provides a significant step forward in understanding the mechanism of NO signaling. |
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Methods | EM (single particle) |
Resolution | 30.0 Å |
Structure data | ![]() EMDB-5861: ![]() EMDB-5862: ![]() EMDB-5863: ![]() EMDB-5864: ![]() EMDB-5865: ![]() EMDB-5866: ![]() EMDB-5867: ![]() EMDB-5868: ![]() EMDB-5869: ![]() EMDB-5870: ![]() EMDB-5871: ![]() EMDB-5872: ![]() EMDB-5873: ![]() EMDB-5874: ![]() EMDB-5875: ![]() EMDB-5876: ![]() EMDB-5877: ![]() EMDB-5878: ![]() EMDB-5879: ![]() EMDB-5880: ![]() EMDB-5881: ![]() EMDB-5882: ![]() EMDB-5883: ![]() EMDB-5884: |
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