EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Single-particle EM reveals the higher-order domain architecture of soluble guanylate cyclase.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 111, Issue 8, Page 2960-2965, Year 2014
掲載日	2014年2月25日
著者	Melody G Campbell / Eric S Underbakke / Clinton S Potter / Bridget Carragher / Michael A Marletta /
PubMed 要旨	Soluble guanylate cyclase (sGC) is the primary nitric oxide (NO) receptor in mammals and a central component of the NO-signaling pathway. The NO-signaling pathways mediate diverse physiological ...Soluble guanylate cyclase (sGC) is the primary nitric oxide (NO) receptor in mammals and a central component of the NO-signaling pathway. The NO-signaling pathways mediate diverse physiological processes, including vasodilation, neurotransmission, and myocardial functions. sGC is a heterodimer assembled from two homologous subunits, each comprised of four domains. Although crystal structures of isolated domains have been reported, no structure is available for full-length sGC. We used single-particle electron microscopy to obtain the structure of the complete sGC heterodimer and determine its higher-order domain architecture. Overall, the protein is formed of two rigid modules: the catalytic dimer and the clustered Per/Art/Sim and heme-NO/O2-binding domains, connected by a parallel coiled coil at two hinge points. The quaternary assembly demonstrates a very high degree of flexibility. We captured hundreds of individual conformational snapshots of free sGC, NO-bound sGC, and guanosine-5'-[(α,β)-methylene]triphosphate-bound sGC. The molecular architecture and pronounced flexibility observed provides a significant step forward in understanding the mechanism of NO signaling.
リンク	Proc Natl Acad Sci U S A / PubMed:24516165 / PubMed Central
手法	EM (単粒子)
解像度	30.0 Å
構造データ	EMDB-5861: Single-particle reconstruction of conformation I of ligand-free sGC (straight) 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5862: Single-particle reconstruction of conformation II of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5863: Single-particle reconstruction of conformation III of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5864: Single-particle reconstruction of conformation IV of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5865: Single-particle reconstruction of conformation V of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5866: Single-particle reconstruction of conformation VI of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5867: Single-particle reconstruction of conformation VII of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5868: Single-particle reconstruction of conformation VIII of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5869: Single-particle reconstruction of conformation IX of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5870: Single-particle reconstruction of conformation X of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5871: Single-particle reconstruction of conformation XI of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5872: Single-particle reconstruction of conformation XII of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5873: Single-particle reconstruction of conformation XIII of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5874: Single-particle reconstruction of conformation XIV of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5875: Single-particle reconstruction of conformation XV of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5876: Single-particle reconstruction of conformation XVI of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5877: Single-particle reconstruction of conformation XVII of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5878: Single-particle reconstruction of conformation XVIII of ligand-free sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5879: Single-particle reconstruction of conformation I of NO-bound sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5880: Single-particle reconstruction of conformation II of NO-bound sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5881: Single-particle reconstruction of conformation I of GPCPP-bound sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5882: Single-particle reconstruction of conformation II of GPCPP-bound sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5883: Single-particle reconstruction of conformation I of GPCPP-bound and NO-bound sGC 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-5884: Single-particle reconstruction of conformation II of GPCPP-bound and NO-bound sGC 手法: EM (単粒子) / 解像度: 30.0 Å
由来	Rattus norvegicus (ドブネズミ)