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-Structure paper
| タイトル | Dynamic adhesion device of phage OE33PA drives Gram-positive host recognition. |
|---|---|
| ジャーナル・号・ページ | bioRxiv, Year 2026 |
| 掲載日 | 2026年5月25日 |
 著者 | Laura Schmitt / Amel Chaïb / Denis Ptchelkine / Eaazhisai Kandiah / Claire Le Marrec / Christian Cambillau / Adeline Goulet /   |
| PubMed 要旨 | Bacteriophages (phages) infecting Gram-positive bacteria must bind to host receptors across thick cell walls to initiate infection, yet the underlying structural mechanisms remain unclear. Here, we ...Bacteriophages (phages) infecting Gram-positive bacteria must bind to host receptors across thick cell walls to initiate infection, yet the underlying structural mechanisms remain unclear. Here, we report cryo-electron microscopy structures of the siphophage OE33PA, providing the first atomic resolution view of a phage infecting this bacterium important for the wine industry. While the overall virion architecture is conserved, the adhesion device displays distinctive features. Its receptor-binding proteins adopt multiple orientations, revealing an intrinsically dynamic assembly. cryo-electron tomography captures distinct conformations upon host attachment, providing rare structural insight into interactions with Gram-positive hosts. Additionally, functional assays show that a highly mobile carbohydrate-binding module in the distal tail protein mediates host-specific binding. Furthermore, the tape measure protein, central to phage assembly and infectivity, adopts a hexameric organization, updating the prevailing trimeric model in siphophages. Together, these findings reveal a dynamic adhesion device in a phage infecting Gram-positive bacteria and highlight the structural and functional diversity of phages. |
 リンク | bioRxiv / PubMed:42244550 / PubMed Central |
| 手法 | EM (トモグラフィー) / EM (単粒子) |
| 解像度 | 2.6 - 4.3 Å |
| 構造データ |  EMDB-57915: Siphohage OE33PA upon binding to its Gram+ host cell surface (view 1)  EMDB-57916: Siphohage OE33PA upon binding to its Gram+ host cell surface (view 2)  EMDB-57918: Siphohage OE33PA upon binding to its Gram+ host cell surface (view 3)  EMDB-57919: Siphohage OE33PA upon binding to its Gram+ host cell surface (view 4)  EMDB-57920: Siphohage OE33PA upon binding to its Gram+ host cell surface (view 5)  EMDB-57921: Siphohage OE33PA upon binding to its Gram+ host cell surface (view 6)  EMDB-57922: Siphohage OE33PA upon binding to its Gram+ host cell surface (view 7) EMDB-58149, PDB-30ys: EMDB-58150, PDB-30yt: EMDB-58151, PDB-30yu: EMDB-58152, PDB-30yv: EMDB-58153, PDB-30yw:  EMDB-58154: DNA in the capsid-tail connector of the phage OE33PA (classification focused on the adaptor-stopper region)  EMDB-58155: DNA in the capsid-tail connector of the phage OE33PA (classification focused on the capsid-portal junction)  EMDB-58156: Adhesion device of the phage OE33PA (unsymmetrized 3D reconstruction) EMDB-58157, PDB-30yx: EMDB-58158, PDB-30yy: EMDB-58159, PDB-30yz: EMDB-58160, PDB-30za:  EMDB-58161: Conformational variability of the phage OE33PA adhesion device (State 1)  EMDB-58162: Conformational variability of the phage OE33PA adhesion device (State 2) |
| 化合物 |  ChemComp-CA: |
| 由来 |
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 キーワード | VIRAL PROTEIN / major capsid protein / siphophage / STRUCTURAL PROTEIN / portal protein / connector assembly / connector / major tail protein / adaptor protein / stopper protein / tail terminator / head to tail connector / Distal tail protein / tail-associated lysin / tape measure protein / distail tail protein / adhesion device / receptor-binding protein / tail tube |
oenococcus phage phioe33pa (ファージ)
Oenococcus oeni (バクテリア)