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-Structure paper
| タイトル | In situ architecture of plasmodesmata in Physcomitrium patens resolved by cryo-electron tomography. |
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| ジャーナル・号・ページ | Nat Plants, Vol. 12, Issue 5, Page 1051-1061, Year 2026 |
| 掲載日 | 2026年5月14日 |
著者 | Marcel Dickmanns / Matthias Pöge / Peng Xu / Sven Gombos / Zoe K Barr / Manuel Miras / Jürgen M Plitzko / Rüdiger Simon / Waltraud X Schulze / Wolf B Frommer / Wolfgang Baumeister / ![]() |
| PubMed 要旨 | Plasmodesmata are nanoscopic channels that traverse plant cell walls, enabling direct intercellular exchange through membrane and cytosolic continuity. Although numerous plasmodesmal components have ...Plasmodesmata are nanoscopic channels that traverse plant cell walls, enabling direct intercellular exchange through membrane and cytosolic continuity. Although numerous plasmodesmal components have been identified, their molecular organization remains poorly defined. Here we used cryo-electron tomography to resolve the in situ architecture of plasmodesmata in Physcomitrium patens across tissues and physiological states. We show how callose-related cell wall remodelling shapes pore architecture to modulate permeability, including a previously undescribed fully sealed state, and resolve helical protein assemblies scaffolding the central, endoplasmic-reticulum-derived desmotubule. Candidate screening via proteomics and structure prediction indicates Multiple C2 Domain and Transmembrane Proteins (MCTPs) as key constituents of these assemblies. In this model, MCTPs tether the desmotubule to the plasma membrane, while their disordered linker regions with polyampholyte charge patterning may populate the cytosolic sleeve. These findings define core architectural features of plasmodesmata and provide a structural framework for understanding how membrane, protein and cell wall components coordinate intercellular connectivity in plants. |
リンク | Nat Plants / PubMed:42135498 / PubMed Central |
| 手法 | EM (トモグラフィー) |
| 構造データ | ![]() EMDB-57163: Cryo-ET of a plasmodesma in wild type Physcomitrium patens gametophore leaflet tissue ![]() EMDB-57164: Cryo-ET of a plasmodesma in wild type Physcomitrium patens protonema tissue ![]() EMDB-57165: Cryo-ET of a plasmodesma in wild type Physcomitrium patens gametophore leaflet tissue ![]() EMDB-57166: Cryo-ET of a plasmodesma in GHL17 Physcomitrium patens protonema tissue ![]() EMDB-57167: Cryo-ET of a plasmodesma in GHL17 Physcomitrium patens protonema tissue ![]() EMDB-57168: Cryo-ET of a plasmodesma in wild type Physcomitrium patens protonema tissue treated with abscisic acid ![]() EMDB-57169: Cryo-ET of a plasmodesma in wild type Physcomitrium patens protonema tissue treated with abscisic acid |
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Physcomitrium patens (植物)