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TitleIn situ architecture of plasmodesmata in Physcomitrium patens resolved by cryo-electron tomography.
Journal, issue, pagesNat Plants, Vol. 12, Issue 5, Page 1051-1061, Year 2026
Publish dateMay 14, 2026
AuthorsMarcel Dickmanns / Matthias Pöge / Peng Xu / Sven Gombos / Zoe K Barr / Manuel Miras / Jürgen M Plitzko / Rüdiger Simon / Waltraud X Schulze / Wolf B Frommer / Wolfgang Baumeister /
PubMed AbstractPlasmodesmata are nanoscopic channels that traverse plant cell walls, enabling direct intercellular exchange through membrane and cytosolic continuity. Although numerous plasmodesmal components have ...Plasmodesmata are nanoscopic channels that traverse plant cell walls, enabling direct intercellular exchange through membrane and cytosolic continuity. Although numerous plasmodesmal components have been identified, their molecular organization remains poorly defined. Here we used cryo-electron tomography to resolve the in situ architecture of plasmodesmata in Physcomitrium patens across tissues and physiological states. We show how callose-related cell wall remodelling shapes pore architecture to modulate permeability, including a previously undescribed fully sealed state, and resolve helical protein assemblies scaffolding the central, endoplasmic-reticulum-derived desmotubule. Candidate screening via proteomics and structure prediction indicates Multiple C2 Domain and Transmembrane Proteins (MCTPs) as key constituents of these assemblies. In this model, MCTPs tether the desmotubule to the plasma membrane, while their disordered linker regions with polyampholyte charge patterning may populate the cytosolic sleeve. These findings define core architectural features of plasmodesmata and provide a structural framework for understanding how membrane, protein and cell wall components coordinate intercellular connectivity in plants.
External linksNat Plants / PubMed:42135498 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-57163: Cryo-ET of a plasmodesma in wild type Physcomitrium patens gametophore leaflet tissue
Method: EM (tomography)

EMDB-57164: Cryo-ET of a plasmodesma in wild type Physcomitrium patens protonema tissue
Method: EM (tomography)

EMDB-57165: Cryo-ET of a plasmodesma in wild type Physcomitrium patens gametophore leaflet tissue
Method: EM (tomography)

EMDB-57166: Cryo-ET of a plasmodesma in GHL17 Physcomitrium patens protonema tissue
Method: EM (tomography)

EMDB-57167: Cryo-ET of a plasmodesma in GHL17 Physcomitrium patens protonema tissue
Method: EM (tomography)

EMDB-57168: Cryo-ET of a plasmodesma in wild type Physcomitrium patens protonema tissue treated with abscisic acid
Method: EM (tomography)

EMDB-57169: Cryo-ET of a plasmodesma in wild type Physcomitrium patens protonema tissue treated with abscisic acid
Method: EM (tomography)

Source
  • Physcomitrium patens (plant)

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