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Structure paper

TitleTranslation initiation by the Kozak mRNA sequence is based on a conformational readout on the ribosome
Journal, issue, pagesTo Be Published
Publish dateMar 2, 2026 (structure data deposition date)
Authorsvon Loeffelholz O / Brachet C / Holvec H / Abour Ramadan A / Protruc C / Maglott-Roth A / de Silva SNT / Hazemann I / Klaholz B
External linksSearch PubMed
MethodsEM (single particle)
Resolution2.9 - 3.2 Å
Structure data

EMDB-56980: focused refined map; 40S head with TC from human 48S PIC on mRNA (non-Kozak)
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-56981: consensus map; human 48S PIC on Kozak mRNA with eIF1A W70A mutant
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-56982: focused refined map; 40S body with eIF1A from human 48S PIC with eIF1A W70A mutant on Kozak mRNA
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-56983: focused refined map; 40S head with TC from human 48S PIC with eIF1A W70A mutant on Kozak mRNA
Method: EM (single particle) / Resolution: 3.2 Å

57004

28zu

EMDB-57004, PDB-28zu:
human 48S PIC with Kozak mRNA and eIF1A W70A mutant
Method: EM (single particle) / Resolution: 3.2 Å

57005

28zv

EMDB-57005, PDB-28zv:
human 48S PIC with mRNA (non-Kozak)
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-57011: focused refined map; 40S body with eIF1A from human 48S PIC on mRNA (non-Kozak)
Method: EM (single particle) / Resolution: 2.95 Å

EMDB-57012: consensus map; human 48S PIC on mRNA (non-Kozak)
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-57015: focused refined map; 40S body with eIF1A from human 48S PIC on Kozak mRNA (Kozak_2)
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-K:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

ChemComp-MET:
METHIONINE

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsRIBOSOME / translation initiation / 48S PIC / human / Kozak sequence

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