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Structure paper

TitleEasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.
Journal, issue, pagesNat. Methods, Year 2026
Publish dateFeb 20, 2026
AuthorsGemin, O. / Armijo, V. / Lecomte, L. / Hons, M. / Deckers, T. / Bissardon, C. / Rossi, C. / Lauzier, K. / Janocha, R. / Felisaz, F. ...Gemin, O. / Armijo, V. / Lecomte, L. / Hons, M. / Deckers, T. / Bissardon, C. / Rossi, C. / Lauzier, K. / Janocha, R. / Felisaz, F. / Sinoir, J. / Linares, R. / Babenko, A. / Kovalev, K. / Prokhorova, I. / Khusainov, I. / Schreiner, C. / Kolesnikova, O. / Salo, V.T. / Schneider, S. / Bowler, M.W. / Wolff, G. / Galej, W.P. / Mahamid, J. / Muller, C.W. / Carugo, K.D. / Eustermann, S. / Mattei, S. / Cipriani, F. / Papp, G.
External linksNat. Methods / PubMed:42337080
MethodsEM (single particle) / EM (subtomogram averaging)
Resolution1.94 - 6.8 Å
Structure data

19434

8rq5

EMDB-19434, PDB-8rq5:
Cryo-EM structure of the light-driven sodium-pumping rhodopsin KR2
Method: EM (single particle) / Resolution: 2.32 Å

EMDB-19880: Cryo-EM structure of human apoferritin (grid prepared with EasyGrid technology)
Method: EM (single particle) / Resolution: 1.94 Å

EMDB-56607: CryoEM structure of guanidinase from Nitrospira inopinata
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-56821: C.t. INO80 chromatin remodeler bound to nucleosome
Method: EM (single particle) / Resolution: 4.93 Å

EMDB-56855: Cryo-EM structure of yeast ribosome solved with EasyGrid
Method: EM (single particle) / Resolution: 2.43 Å

EMDB-56861: In situ ribosome from HeLa cells
Method: EM (subtomogram averaging) / Resolution: 6.8 Å

Chemicals

ChemComp-OLC:
(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-LFA:
EICOSANE

ChemComp-RET:
RETINAL

ChemComp-NA:
Unknown entry

ChemComp-OLA:
OLEIC ACID

ChemComp-HOH:
WATER

Source
  • dokdonia eikasta (bacteria)
  • Homo sapiens (human)
  • Candidatus Nitrospira inopinata (bacteria)
  • Thermochaetoides thermophila (fungus)
  • Schizosaccharomyces pombe 972h- (yeast)
KeywordsMEMBRANE PROTEIN / retinal / ion transport / sodium / rhodopsin / photocycle / pentamer

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