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TitleAdaptations in Plasmodium tubulin determine distinct microtubule architectures, mechanics and drug susceptibility.
Journal, issue, pagesNat Commun, Vol. 17, Issue 1, Year 2026
Publish dateMar 5, 2026
AuthorsMamata Bangera / Jiangbo Wu / Daniel Beckett / Dominik Fachet / Josie L Ferreira / Gregory A Voth / Simone Reber / Carolyn A Moores /
PubMed AbstractMicrotubules are ubiquitous yet diverse cytoskeleton filaments. However, tubulin conservation presents challenges in understanding the origins of diverse microtubule architectures. The mechanisms by ...Microtubules are ubiquitous yet diverse cytoskeleton filaments. However, tubulin conservation presents challenges in understanding the origins of diverse microtubule architectures. The mechanisms by which microtubule architecture varies through the life cycle of the malaria-causing parasite Plasmodium are not understood and provide a valuable framework for exploring how intrinsic properties of tubulin contribute to architectural variety. Using parasite-purified tubulin, we determine P. falciparum microtubule structures by cryo-electron microscopy. Parasite-specific sequences change the tubulin dimer structure, suggesting how drug susceptibility and polymer properties are modified. Within the P. falciparum microtubule, lateral contacts are smaller but stronger, and the lattice is stiffer than in brain microtubules. Non-canonical microtubule architectures found in parasites are highly similar to those observed in vitro, validating the physiological relevance of these properties. Our findings show how evolutionary adaptation of tubulin modulates the material properties of the microtubule cytoskeleton.
External linksNat Commun / PubMed:41786731 / PubMed Central
MethodsEM (helical sym.) / EM (subtomogram averaging)
Resolution2.9 - 25.0 Å
Structure data

53571

9r4x

EMDB-53571, PDB-9r4x:
13 protofilament P. falciparum paclitaxel stabilised GDP microtubule
Method: EM (helical sym.) / Resolution: 3.2 Å

53572

9r4y

EMDB-53572, PDB-9r4y:
15 protofilament P. falciparum GMPCPP microtubule
Method: EM (helical sym.) / Resolution: 2.9 Å

EMDB-56294: Plasmodium falciparum gametocyte microtubule with 15 protofilaments determined in situ
Method: EM (subtomogram averaging) / Resolution: 25.0 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

Source
  • plasmodium falciparum 3d7 (eukaryote)
KeywordsSTRUCTURAL PROTEIN / Malaria / parasite / cytoskeleton / microtubule / paclitaxel / GMPCPP

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