| Title | Structural mechanisms of unidirectional prophage integration and excision by serine integrases |
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| Journal, issue, pages | To Be Published |
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| Publish date | Feb 12, 2025 (structure data deposition date) |
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Authors | Sun YE / Aspinall L / Joseph AP / Colloms SD / Stark WM / Spagnolo L |
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External links | Search PubMed |
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| Methods | EM (single particle) |
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| Resolution | 3.0 - 7.23 Å |
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| Structure data | EMDB-52811, PDB-9ibl: PhiC31 integrative synapse: attB x attP with an S12A integrase variant Method: EM (single particle) / Resolution: 7.21 Å EMDB-52845, PDB-9ifc: PhiC31 integrative synapse: CC dimer subregion Method: EM (single particle) / Resolution: 3.79 Å EMDB-53874, PDB-9rad: PhiC31 integrase-attL complex: B-bound subregion Method: EM (single particle) / Resolution: 3.36 Å EMDB-53875, PDB-9rae: PhiC31 integrase-attL complex: P'-bound subregion Method: EM (single particle) / Resolution: 3.88 Å EMDB-53878, PDB-9rah: Autoinhibited PhiC31 integrase-attL complex Method: EM (single particle) / Resolution: 4.37 Å EMDB-55829, PDB-9teh: PhiC31 excisive synapse; attL x attL with an integraseS12A-RDF protein fusion Method: EM (single particle) / Resolution: 7.23 Å EMDB-55830, PDB-9ten: PhiC31 excisive synapse: B-bound subregion Method: EM (single particle) / Resolution: 3.11 Å EMDB-55831, PDB-9teo: PhiC31 excisive synapse: P'-bound subregion Method: EM (single particle) / Resolution: 3.27 Å EMDB-55832, PDB-9tep: PhiC31 integraseS12A-attB-RDF complex Method: EM (single particle) / Resolution: 3.67 Å EMDB-55833, PDB-9teq: PhiC31 integraseS12A-attB-RDF complex: B-bound subregion Method: EM (single particle) / Resolution: 3.0 Å EMDB-55834, PDB-9ter: PhiC31 integraseS12A-attB-RDF complex: B'-bound subregion Method: EM (single particle) / Resolution: 3.13 Å |
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| Chemicals | |
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| Source | lomovskayavirus c31
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Keywords | DNA BINDING PROTEIN / Serine integrase / Recombinase / Site-specific DNA recombination / Bacteriophage / RECOMBINATION / Site-specific recombination / RDF / DNA |
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