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| Title | Influence of total electron dose on the quality of nucleic acids potential maps in Cryo-EM. |
|---|---|
| Journal, issue, pages | Ultramicroscopy, Vol. 283, Page 114358, Year 2026 |
| Publish date | Mar 21, 2026 |
 Authors | Grzegorz Ważny / Marcin Jaciuk / Paulina Indyka / Sebastian Glatt / Artur Biela / Michał Rawski /   |
| PubMed Abstract | The standardization of protocols in science lies at the basis of every experiment. During this process, some crucial questions need to be answered. The quality of results in single particle cryo- ...The standardization of protocols in science lies at the basis of every experiment. During this process, some crucial questions need to be answered. The quality of results in single particle cryo-Electron Microscopy (cryo-EM) requires optimized data collection parameters. Hence, before each experiment, the following questions need to be defined: (i) which optimal electron dose should be used? (ii) at which level does the accumulated electron damage start to affect resolution? (iii) how does pre-exposure influence the level of damage? (iv) how does dose weighting over the scale of total electron dose deposited to the sample influence the resulting reconstruction? In this manuscript, we addressed all of the above questions by performing a systematic analysis of the relation between data collection strategies and the resulting outcome in the form of cryo-EM map quality of Escherichia coli and H. sapiens ribosome particles. Our findings will serve as references for users seeking to optimize data collection and reconstruction routines for single particle cryo-EM experiments. |
 External links | Ultramicroscopy / PubMed:41886978 |
| Methods | EM (single particle) |
| Resolution | 2.28 - 7.23 Å |
| Structure data |  EMDB-55665: E.coli ribosome reconstruction from frames 1-20, dose weighted  EMDB-55666: E.coli ribosome reconstruction from frames 21-40, dose weighted  EMDB-55667: E.coli ribosome reconstruction from frames 41-60, dose weighted  EMDB-55668: E.coli ribosome reconstruction from frames 61-80, dose weighted  EMDB-55669: E.coli ribosome reconstruction from frames 1-40, dose weighted  EMDB-55670: E.coli ribosome reconstruction from frames 1-80, dose weighted  EMDB-55671: E.coli ribosome reconstruction from frames 1-20, not dose weighted  EMDB-55672: E.coli ribosome reconstruction from frames 1-40, not dose weighted  EMDB-55673: E.coli ribosome reconstruction from frames 1-80, not dose weighted  EMDB-55674: human type ribosome reconstruction from frames 1-20  EMDB-55675: human type ribosome reconstruction from frames 21-40  EMDB-55676: human type ribosome reconstruction from frames 41-60  EMDB-55677: human type ribosome reconstruction from frames 61-80  EMDB-55678: human type ribosome reconstruction from frames 1-40  EMDB-55679: human type ribosome reconstruction from frames 1-80  EMDB-55680: human type ribosome reconstruction from frames 1-20, not dose weighted  EMDB-55681: human type ribosome reconstruction from frames 1-40, not dose weighted  EMDB-55682: human type ribosome reconstruction from frames 1-80 |
| Source |
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Escherichia coli (E. coli)
Homo sapiens (human)