Movie
Controller
Structure viewers
About Yorodumi Papers
+Search query
-Structure paper
| Title | Structure of the 30S translation initiation complex coupled to paused RNA polymerase and its potential for riboregulation. |
|---|---|
| Journal, issue, pages | Nat Commun, Vol. 17, Issue 1, Page 693, Year 2025 |
| Publish date | Dec 13, 2025 |
 Authors | Johann J Roske / Giulia Paris / Akanksha Goyal / Marina Rodnina / Nikolay Zenkin / Katarzyna J Bandyra / Ben F Luisi /    |
| PubMed Abstract | In many bacterial species, transcription and translation can be coupled physically, with potential impact on the rates and efficiency of gene expression. Here, we present structural evidence from ...In many bacterial species, transcription and translation can be coupled physically, with potential impact on the rates and efficiency of gene expression. Here, we present structural evidence from cryo-EM demonstrating that a bacterial RNA polymerase that is paused proximally to the promoter can associate with the pioneering 30S translation initiation complex (30S IC). These findings suggest that the physical link between transcription and translation can be established prior to commitment to protein synthesis. Although the mRNA is embedded in this 'early expressome' complex, it can nonetheless interact with small regulatory RNA (sRNA) and be targeted for cleavage in the protein-coding region by the RNA degradosome assembly in vitro. The potential tagging of transcripts with sRNA during pioneering and subsequent stages of translation initiation, when the 30S IC is at the 5' end of a polyribosome, may in principle contribute to efficient and rapid termination of gene expression in response to regulatory signals. |
 External links | Nat Commun / PubMed:41390511 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 3.0 - 5.8 Å |
| Structure data |  EMDB-55527: Early Expressome Composite Map of TEC and 30S IC  EMDB-55528: Early Expressome Consensus Refinement  EMDB-55529: Early Expressome RNAP/TEC body  EMDB-55530: E. coli 30S IC containing mRNA, IF1 and IF3  EMDB-55531: E. coli 30S IC containing mRNA, initiator tRNA, IF1 and IF2 |
| Source |
|
Escherichia coli (E. coli)